Amino Acid Sequence and Characterization of a Rice Bran Ribonuclease
スポンサーリンク
概要
- 論文の詳細を見る
A base-nonspecific and acid ribonuclease(RNase Os)belonging to the RNase T2 family was purified from rice bran to a homogeneous state by SDS-PAGE. The primary structure of RNase Os was determined by protein chemistry and molecular cloning. The RNase Os was a simple protein and consisted of 205 amino acid residues. Its molecular weight was 22578 and its amino acid sequence showed that it was most similar to barley RNase among the known RNase T2 family enzymes having 157 amino acid residues identical with barley RNase. However, its N-terminus was blocked by a γ-pyroglutamyl residue. The optimal pH of RNase Os was around 5.5. The base preference at the B1 and B2 site of RNase Os was estimated from the rates of hydrolysis of 16 dinucleoside phosphates, to be guanine as the case of RNase LE from tomato. RNase Os was successfully expressed from yeast cells using the E.coli/yeast expression vector pYE-RNAP.
- 社団法人日本薬学会の論文
- 2001-07-01
著者
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Koyama Takashi
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
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Koyama Takashi
Division Of Life Sciences Graduate School Of Natural Science And Technology Kanazawa University
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ogawa Y
Department Of Applied Microbiology Hoshi University
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Ogawa Yuko
School Of Pharmaceutical Sciences Mukogawa Women's University
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TSUJI Tsutomu
Department of Legal Medicine, Wakayama Medical University
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie M
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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Koyama Takashi
Department Of Microbiology College Of Pharmacy Nihon University
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ITAGAKI Tadashi
Department of Microbiology, College of Pharmacy, Nihon University
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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INOKUCHI Norio
Department of Microbiology, College of Pharmacy, Nihon University
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OGAWA Yuko
Department of Microbiology, Hoshi College of Pharmacy
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YAMAGISHI Minako
Department of Microbiology, Hoshi College of Pharmacy
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IMAI Ryozo
Department of Low Temperature Sciences, Hokkaido National Agriculture Experimental Station
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Ogawa Yuko
Department Of Applied Microbiology Hoshi University
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Yamagishi M
Department Of Microbiology Hoshi College Of Pharmacy
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Itagaki Tadashi
Department Of Microbiology College Of Pharmacy Nihon University
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Iwama M
Department Of Microbiology Faculty Of Pharmaceutical Sciences Ohu-university
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Imai Ryozo
Department Of Low Temperature Sciences Hokkaido National Agriculture Experimental Station
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Tsuji T
Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Tsuji Tsutomu
Department Of Legal Medicine Wakayama Medical University
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Inokuchi N
Department Of Microbiology College Of Pharmacy Nihon University
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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Koyama Takashi
Department of Cardiology, Akita University School of Medicine
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