Enzymatic Activities of Several K108 Mutants of Ribonuclease (RNase) Rh Isolated from Rhizopus niveus
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概要
- 論文の詳細を見る
We previously investigated the role of the Lys108 residue of ribonuclease (RNase) Rh from Rhizopus niveus, and suggested that Lys108 probably acts to stabilize the pentacovalent intermediate, and that an Arg residue could replace the role of Lys108. In RNase Le2 from Lentinus edodes, a homologous enzyme of RNase Rh, Lys108 is replaced by Thr. In this paper, the enzymatic properties of a K108T mutant and its analogous enzyme, K108S, were investigated to determine the effect of Thr and its analog, Ser at the 108th position on enzyme activity. The enzymatic properties of these mutant enzymes were compared with those of other mutant enzymes at this position (K108M, K108A, K108L). The results showed that Thr and Ser could replace Lys108 but resulted in only 2-20% of the activity of the native enzyme depending on the substrates used.
- 公益社団法人日本薬学会の論文
- 1996-08-15
著者
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ogawa Y
Department Of Applied Microbiology Hoshi University
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Ogawa Yuko
School Of Pharmaceutical Sciences Mukogawa Women's University
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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OGAWA Yuko
Department of Microbiology, Hoshi College of Pharmacy
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Ogawa Yuko
Department Of Applied Microbiology Hoshi University
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HAGIWARA Chinatsu
Department of Microbiology, Hoshi College of Pharmacy
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ONO Eriko
Department of Microbiology, Hoshi College of Pharmacy
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KAWAGUCHI Ryoko
Department of Microbiology, Hoshi College of Pharmacy
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KANAZAWA Chiharu
Department of Microbiology, Hoshi College of Pharmacy
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Ono Eriko
Department Of Microbiology Hoshi College Of Pharmacy
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Ono Eriko
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Kanazawa Chiharu
Department Of Microbiology Hoshi College Of Pharmacy
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Kawaguchi Ryoko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Hagiwara Chinatsu
Department Of Microbiology Hoshi College Of Pharmacy
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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