Enzymatic Properties of Sialic Acid Binding Lectin from Rana catesbeiana Modified with a Water-Soluble Carbodiimide in the Presence of Various Nucleophiles

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概要

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• The anti-tumor activity of sialic acid binding lectin from Rana catesbeiana(cSBL)was increased by chemical modification with a water-soluble carbodiimide(EDC)in the presence of nueleophiles such as ethylenediamine and glycine methylester. lnvestigations on ribonuclease(RNase)activities of the modified cSBLs were conducted to elucidate the fundamental mechanisms underlying enhancement of the anti-tumor activity conferred by these modifications. The following three characteristics were observed with modification.(i)RNase aetivitv of the modified eSBL was enhanced towards double stranded RNA and RNA-oligo dA hybrids. The activity increase was observed even under physiologic ionic strength conditions;(ii)RNase activity of the modified cSBL towards single stranded RNA and poly U decreased, while the activity towards poly C was unaffected;(iii)the base preference of the B2 base recognition site of modified cSBL decreased for guanine. On the contrary, the preference for cytosine and adenine increased. This result may explain why the RNase activity towards poly C was not affected by EDC-modification as mentioned above.

• 公益社団法人日本薬学会の論文
• 2001-12-01

著者

• Irie Masachika

  Department of Microbiology, Hoshi College of Pharmacy

• Ohgi Kazuko

  Department of Microbiology, Hoshi College of Pharmacy

• Ogawa Y

  Department Of Applied Microbiology Hoshi University

• Ogawa Yuko

  School Of Pharmaceutical Sciences Mukogawa Women's University

• TSUJI Tsutomu

  Department of Legal Medicine, Wakayama Medical University

• Ohgi K

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Ohgi Kazuko

  Department Of Microbiology Hoshi College Of Pharmacy

• Irie M

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Irie Masachika

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Irie Masachika

  Department Of Microbiology Hoshi College Of Pharmacy

• IWAMA Masanori

  Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University

• OGAWA Yuko

  Department of Microbiology, Hoshi College of Pharmacy

• Ogawa Yuko

  Department Of Applied Microbiology Hoshi University

• Irie M

  Hoshi Coll. Pharmacy Tokyo Jpn

• Iwama M

  Department Of Microbiology Faculty Of Pharmaceutical Sciences Ohu-university

• Tsuji T

  Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Tsuji Tsutomu

  Department Of Legal Medicine Wakayama Medical University

• Ohgi Kazuko

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Ohgi K

  Department Of Microbiology Hoshi College Of Pharmacy

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