A Protease Sensitive Region of Plant and Animal Ribonucleases Belonging to the RNase T2 Family
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概要
- 論文の詳細を見る
Recombinant RNase LE from tomato and squid liver RNase Tp, typical plant/animal type RNases belonging to the RNase T2 family, were subjected to limited digestion with several proteases, and the cleavage sites were analyzed by Edman degradation. Recombinant RNase LE was cleaved specifically at the 24th Lys by lysylen-dopeptidase and trypsin, and RNase Tp was cleaved at the 21st Glu by V8 protease. These cleavage sites are located very close to those where the cleavage during preparation of several animal RNase T2 family enzymes was observed. From this finding, it was concluded that the short segment around the 20th amino acid residue in plant/animal RNases in located on the surface of the molecules and forms loops, and is thus very sensitive to proteases.
- 公益社団法人日本薬学会の論文
- 1998-06-15
著者
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ogawa Y
Department Of Applied Microbiology Hoshi University
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Ogawa Yuko
School Of Pharmaceutical Sciences Mukogawa Women's University
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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OGAWA Yuko
Department of Microbiology, Hoshi College of Pharmacy
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Ogawa Yuko
Department Of Applied Microbiology Hoshi University
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KUSANO Ayumu
Ohhashi Hospital, Toho University
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Kusano Ayumu
Ohashi Hospital Toho University
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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