Distribution of a Kidney Acid-ribonuclease-like Enzyme and the Other Ribonucleases in Bovine Organs and Body Fluids
スポンサーリンク
概要
- 論文の詳細を見る
To determine the distribution of a kidney acid RNase (RNase K2) and other RNases, the levels of RNase K2, RNase A, and seminal RNase (RNase Vs1) in bovine tissues and body fluids were measured by enzyme immunoassay. The crude extracts of several tissues and body fluids were fractionated by phospho-cellulose column chromatography. The enzymatic activities at pH 7.5 and 6.0 and enzyme contents of each tube were measured by enzyme assay and enzyme immunoassay, respectively. In the pancreas, parotid gland, and heart, most RNase activity was due to a single peak of RNase A, but a small amount of RNase K2 was always observed. In the kidney, there was about 5 times as much RNase K2 as RNase A. In the lung, although RNase K2 and RNase A were the major components, there are another two alkaline RNase peaks. In the spleen and liver, there are four RNases, two acid RNases, one of which is RNase K2, and two alkaline RNases including RNase A. A new acid RNase (non RNase K2-acid RNase) from both organs was immunologically the same. In serum, there are at least four RNases. By partial purification of serum RNases by phosphocellulose and heparin-Sepharose column chromatographies, at least 4 RNases, RNase A, RNase K2 and the other two alkaline RNases, one of which is immunologically indistinguishable from liver alkaline RNase, were confirmed. The other serum alkaline RNase was immunologically related to lung and spleen alkaline RNases. In conclusion, in bovine tissues and body fluids there are at least 7 types of pyrimidine-base-specific RNases: brain RNase, seminal RNase, RNase A, RNase K2, an acid RNase (RNase BSP1), an alkaline RNase (RNase BL4), and another alkaline RNase in serum.
- 社団法人 日本農芸化学会の論文
著者
-
Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
-
Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
SANDA Akihiro
Faculty of Public Health, Azabu University
-
Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
MORITA Tom
Department of Microbiology, Hoshi College of Pharmacy
-
TAKIZAWA Yoshio
Faculty of Public Health, Azabu University
関連論文
- Base Specificity of Two Plant Seed Ribonucleases from Momoridica charantia and Luffa cylindrica
- Primary Structure and Properties of Ribonuclease Bm2 (RNase Bm2) from Bryopsis maxima(Biochemistry)
- Amino Acid Sequence and Characterization of a Nuclease (Nuclease Le3) from Lentinus edodes(Biochemistry & Molecular Biology)
- Amino Acid Sequence of a Trypsin Inhibitor from a Spirometra(Spirometra erinaceieuropaei)
- Amino Acid Sequence and Characterization of a Rice Bran Ribonuclease
- Relationship to Two Ribonucleases with Molecular Masses of 45 kDa and 37 kDa from the Culture Medium of Lentinus edodes
- Amino Acid Sequence of an Unique Ribonuclease with a C-Terminus rich in O-Glycosylated Serine and Threonine from Culture Medium of Lentinus edodes
- Comparison of Base Specificity and Other Enzymatic Properties of Two Protozoan Ribonucleases from Physarum polycephalum and Dictyostelium discoideum
- Characterization and Primary Structure of a Base Non-Specific and Acid Ribonuclease from Dictyostelium discoideum
- Characterization of Ribonucleases from Culture Medium of Lentinus edodes
- Primary Structure of Base Non-specific and Acid Ribonuclease from Bullfrog (Rana catesbeiana)
- The Base Specificities of Tomato Ribonuclease (RNase LE) and Its Asp44 Mutant Enzyme Expressed from Yeast Cells
- Purification and Characterization of the 2nd 5'-Nucleotide-forming Nuclease from Lentinus edodes
- Primary Structure of a Base Non-specific and Adenylic Acid Preferential Ribonuclease from the Fruit Bodies of Lentinus edodes
- Purification and Characterization of a Base Non-specific and Adenylic Acid Preferring Ribonuclease from the Fruit Bodies of Lentinus edodes(Biological Chemistry)
- The Role of the Carboxyl Group of Asp_ of Bovine Pancreatic Ribonuclease A in Enzymatic Activity(Biological Chemistry)
- Effect of Modification of the Carboxyl Groups of the Sialic Acid Binding Lectin from Bullfrog(Rana catesbeiana)Oocyte on Anti-tumor Activity
- Amino Acids and Peptides. LV. Application of 2-Adamantyl Dericatives as Protecting Groups to the Synthesis of Peptide Fragments Related to Sulfolobus solifataricus Ribonuclease. II
- Amino Acids and Peptides. LIV. Application of 2-Adamantyl Derivatives as Protecting Groups to the Synthesis of Peptide Fragments Related to Sulfolobus solifataricus Ribonuclease. I
- Role of Lys108 in the Enzymatic Activity of RNase Rh from Rhizopus niveus
- Primary Structure, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from bacillus stearothermophilus
- On a Salmon (Onchorhynchus keta) Liver RNase, Belonging to RNase T2 Family : Primary Structure and Some Properties
- Effect of Replacing the Aspartic Acid / Glutamic Acid Residues of Bullfrog Sialic Acid Binding Lectin with Asparagine / Glutamine and Arginine on the Inhibition of Cell Proliferation in Murine Leukemia P388 Cells
- Enzymatic Properties of Sialic Acid Binding Lectin from Rana catesbeiana Modified with a Water-Soluble Carbodiimide in the Presence of Various Nucleophiles
- A Protease Sensitive Region of Plant and Animal Ribonucleases Belonging to the RNase T2 Family
- Enzymatic Activities of Several K108 Mutants of Ribonuclease (RNase) Rh Isolated from Rhizopus niveus
- Expression of Bacillus Protease (Protease BYA) from Bacillus sp. Y in Bacillus subtilis and Enhancement of Its Specific Activity by Site-Directed Mutagenesis : Improvement in Productivity of Detergent Enzyme(Molecular and Cell Biology)
- Purification and Properties of a Novel Surface-active Agent- and Alkaline-resistant Protease from Bacillus sp. Y(Biological Chemistry)
- Marked Changes in the Ribonuclease Activity of Mature and Immature Gonads of Sea Urchins Hemicentrotus pulcherrimus and Anthocidaris crassispina(Biochemistry)
- Enzymatic Properties of Serine 93 Mutants of RNase Rh from Rhizopus niveus. : A Trial to Alter the Base Preference of RNase Rh(Biochemistry)
- A New Type of RNase T2 Ribonuclease in Two Basidiomycetes Fungi, Lentinus edodes and Irpex lacteus(Biochemistry & Molecular Biology)
- Enzymatic Properties of Glutamine 32 Mutants of RNase Rh from Rhizopus niveus, a Trial to Alter the Most Preferential Inter-nucleotidic Linkages of RNase Rh(Biochemistry & Molecular Biology)
- Enzymatic Properties of Phenylalanine 101 Mutant Enzyme of Ribonuclease Rh from Rhizopus niveus
- Enzymatic Properties of Double Mutant Enzymes at Asp51 and Trp49 and Asp51 and Tyr57 of RNase Rh from Rhizopus niveus
- Enzymatic Properties of Mutant Forms of RNase Rh from Rhizopus niveus as to Asp51^1
- Enzymatic Properties of Mutant Enzymes at Trp49 and Tyr57 of RNase Rh from Rhizopus niveus ^1
- Primary Structure of a Nuclease (Nuclease PA3) from a Penicillium sp.(Biological Chemistry)
- Bacteriolytic Activity of Alkaline Protease BYA from Bacillus sp. Y
- Base Non-specific Acid Ribonuclease from Irpex lacteus, Primary Structure and Phylogenetic Relationships in RNase T_2 Family Enzyme
- Base Specificity and Primary Structure of Poly U-preferential Ribonuclease from Chicken Liver
- Identification of the Glutamyl and Histidyl Residues Involved in the Active Site of a Minor Ribonuclease from Aspergillus saitoi(Biological Chemistry)
- Role of Histidine 46 in the Hydrolysis and the Reverse Transphosphorylation Reaction of RNase Rh from Rhizopus niveus^1
- Primary Structure of a Squid Acid and Base Non-specific Ribonuclease
- Purification and Primary Structure of a Porcine Kidney Non-secretory Ribonuclease
- Distribution of a Kidney Acid-ribonuclease-like Enzyme and the Other Ribonucleases in Bovine Organs and Body Fluids
- Enzymatic Properties of Mutant Enzymes at Trp49 and Tyr57 of RNase Rh from Rhizopus niveus.
- Enzymatic Properties of Mutant Forms of RNase Rh from Rhizopus niveus as to Asp51.