Amino Acid Sequence and Characterization of a Nuclease (Nuclease Le3) from Lentinus edodes(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
The fruit body of shiitake (Lentinus edodes) produces two acid nucleases, nuclease Le1 and nuclease Le3, both of which are thought to be candidates for the enzyme that produces a flavorful substance, 5'-GMP, and the primary structure of one of the nucleases, nuclease Le1, has been analyzed by both protein chemistry and gene cloning [Biosci. Biotechnol. Biochem. 64, 948-957 (2000)]. In this study the amino acid sequence of nuclease Le3 was analyzed by protein chemistry and gene cloning. Nuclease Le3 is a glycoprotein that contains 280 amino acid residues, and the molecular mass of the protein moiety of nuclease Le3 is 31,045. The nucleotide sequence of the cDNA and genomic DNA encoding nuclease Le3 revealed the presence of an 18-residue putative signal peptide. Nuclease Le3 contains 170, 108, and 98 amino acid residues that are identical to residues of nuclease Le1, nuclease P1, and nuclease S, respectively. The amino acid residues involved in coordination with Zn^<2+> atoms in nuclease P1 are all conserved in nuclease Le3. Nuclease Le3 contains 9 half-cystine residues, and 7 of them are located in the same positions as in nuclease Le1.
- 社団法人日本農芸化学会の論文
- 2002-06-23
著者
-
Koyama Takashi
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
-
Kobayashi H
National Food Research Institute
-
Koyama Takashi
Division Of Life Sciences Graduate School Of Natural Science And Technology Kanazawa University
-
Kobayashi H
National Food Res. Inst. Ibaraki Jpn
-
Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
-
Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
-
Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
-
Irie M
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
-
Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
-
Hayashi K
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
-
KOBAYASHI Hiroko
Department of Internal Medicine II, Fukushima Medical University School of Medicine
-
KUMAGAI Fumi
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
-
Koyama Takashi
Department Of Microbiology College Of Pharmacy Nihon University
-
ITAGAKI Tadashi
Department of Microbiology, College of Pharmacy, Nihon University
-
IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
-
INOKUCHI Norio
Department of Microbiology, College of Pharmacy, Nihon University
-
Itagaki Tadashi
Department Of Microbiology College Of Pharmacy Nihon University
-
Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
-
Kumagai F
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
-
Kumagai Fumi
Dept. Int. Biosci. Grad. Sch. Front. Sci. Tokyo Univ.
-
Kumagai Fumi
Department Of Microbiology College Of Pharmacy Nihon University
-
Kumagai Fumi
Department Of Integrated Biosciences Graduate School Of Frontier Sciences The University Of Tokyo
-
Iwama M
Department Of Microbiology Faculty Of Pharmaceutical Sciences Ohu-university
-
Inokuchi N
Department Of Microbiology College Of Pharmacy Nihon University
-
Kobayashi Hiroko
Department Of Gastroenterology And Rheumatology Fukushima Medical University School Of Medicine
-
Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
-
Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
-
Koyama Takashi
Department of Cardiology, Akita University School of Medicine
-
Kobayashi Hiroko
Department of Cardiovascular Disease Research, Eisai Tsukuba Research Laboratories
関連論文
- Beneficial Effects of Adaptive Servo Ventilation in Patients With Chronic Heart Failure
- Screening for Milk-clotting Enzyme from Basidiomycetes
- Deodorization of Soybean Protein with Microorganisms
- Action of Soymilk-clotting Enzyme from Bacillus sp. K-295G-7 on the Acidic Subunit of Soybean 11S Globulin(Biological Chemistry)
- Cloning and Sequence Analysis of cDNA for Irpex lacteus Aspartic Proteinase(Biological Chemistry)
- Purification and Characterization of Extracellular Poly(β-D-1, 4-mannuronide) Lyase from Dendryphiella salina IFO 32139
- Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With αMyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
- Beneficial Effects of Adaptive Servo Ventilation in Patients With Chronic Heart Failure
- Base Specificity of Two Plant Seed Ribonucleases from Momoridica charantia and Luffa cylindrica
- Adnexal Masses Caused by Pelvic Inflammatory Disease : MR Appearance
- Paraneoplastic syndromes associated with ovarian neoplasms
- Crystallization and Preliminary X-ray Crystallographic Study of Streptomyces olivaceoviridis E-86 β-Xylanase
- Screening for Phenoloxidases from Edible Mushrooms(Biochemistry & Molecular Biology)
- Purification and Characterization of an α-L-Arabinofuranosidase from suspension cultured Non-embryo genic carrot (Daucus carota. L)
- PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
- Substrate Specificity of the α-L-Arabinofuranosidase from Trichoderma reesei
- Substrate Specificites of α-L-Arabinofuranosidases Produced by Two Species of Aspergillus niger
- Purification and Characterization of α-Glucuronidase from Snail Acetone Powder
- Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With αMyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
- OJ-019 The Percentage of Angiogenic T Cells in Peripheral Blood was Increased in the Patients with Coronary Artery Disease(OJ04,Angina Pectoris (Basic, Clinical) (IHD),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circula
- FRS-055 Causative Relationship between Cor Pulmonale and Atrial Arrhythmia : Novel Insight into Right Atrial Electrical Remodeling(FRS11,New Insights into Basic Mechanisms of Arrhythmia (A),Featured Research Session (English),The 73rd Annual Scientific Me
- Molecular and Electrical Remodeling of L- and T-Type Ca^ Channels in Rat Right Atrium With Monocrotaline-Induced Pulmonary Hypertension(Arrhythmia/Electrophysiology)
- Molecular and Electrical Remodeling of L- and T-Type Ca2+ Channels in Rat Right Atrium With Monocrotaline-Induced Pulmonary Hypertension
- PJ-317 Cilnidipine, L/N-type Calcium Channel Antagonist, Improves Cardiac Sympathetic Activity and Left Ventricular Diastolic Function in Patients With Hypertensive Heart Disease(Cardiac hypertrophy, basic/clinical-3, The 71st Annual Scientific Meeting of
- Kinetic Analysis of Temperature-Programmed Autoxidation of Ethyl Eicosapentaenoate and Ethyl Docosahexaenoate
- Kinetic Analysis of the Autoxidation of Ethyl Eicosapentaenoate at Different Oxygen Levels
- Primary Structure and Properties of Ribonuclease Bm2 (RNase Bm2) from Bryopsis maxima(Biochemistry)
- Amino Acid Sequence and Characterization of a Nuclease (Nuclease Le3) from Lentinus edodes(Biochemistry & Molecular Biology)
- Amino Acid Sequence of a Trypsin Inhibitor from a Spirometra(Spirometra erinaceieuropaei)
- Amino Acid Sequence and Characterization of a Rice Bran Ribonuclease
- Relationship to Two Ribonucleases with Molecular Masses of 45 kDa and 37 kDa from the Culture Medium of Lentinus edodes
- Amino Acid Sequence of a Nuclease (Nuclease Le1) from Lentinus edodes
- Amino Acid Sequence of an Unique Ribonuclease with a C-Terminus rich in O-Glycosylated Serine and Threonine from Culture Medium of Lentinus edodes
- Comparison of Base Specificity and Other Enzymatic Properties of Two Protozoan Ribonucleases from Physarum polycephalum and Dictyostelium discoideum
- Characterization and Primary Structure of a Base Non-Specific and Acid Ribonuclease from Dictyostelium discoideum
- Characterization of Ribonucleases from Culture Medium of Lentinus edodes
- Primary Structure of Base Non-specific and Acid Ribonuclease from Bullfrog (Rana catesbeiana)
- The Base Specificities of Tomato Ribonuclease (RNase LE) and Its Asp44 Mutant Enzyme Expressed from Yeast Cells
- Purification and Characterization of the 2nd 5'-Nucleotide-forming Nuclease from Lentinus edodes
- Primary Structure of a Base Non-specific and Adenylic Acid Preferential Ribonuclease from the Fruit Bodies of Lentinus edodes
- Purification and Characterization of a Base Non-specific and Adenylic Acid Preferring Ribonuclease from the Fruit Bodies of Lentinus edodes(Biological Chemistry)
- PJ-074 The Prevalence of Asymptomatic Apical Asynergy in Patients with Long-term Right Ventricular Apical Pacing(PJ013,Cardiac Function (Clinical) 1 (M),Poster Session (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- The Role of the Carboxyl Group of Asp_ of Bovine Pancreatic Ribonuclease A in Enzymatic Activity(Biological Chemistry)
- Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
- Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
- Characterization of a Thermostable Endo-β-1,4-D-galactanase from the Hyperthermophile Thermotoga maritima
- Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
- Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
- Effect of Modification of the Carboxyl Groups of the Sialic Acid Binding Lectin from Bullfrog(Rana catesbeiana)Oocyte on Anti-tumor Activity
- Purification, Characterization, and cDNA Cloning of a Novel α-Galactosidase from Mortierella vinacea
- Purification and Some Properties of α-Galactosidase from Penicillium purpurogenum
- Biogenesis of 2-Phenylethanol in Rose Flowers : Incorporation of [^2H_8]L-Phenylalanine into 2-Phenylethanol and its β-D-Glucopyranoside during the Flower Opening of Rosa 'Hoh-Jun' and Rosa damascena Mill.(Food & Nutrition Science)
- Substrate Specificity of β-Primeverosidase, A Key Enzyme in Aroma Formation during Oolong Tea and Black Tea Manufacturing(Food & Nutrition Science)
- Amino Acids and Peptides. LV. Application of 2-Adamantyl Dericatives as Protecting Groups to the Synthesis of Peptide Fragments Related to Sulfolobus solifataricus Ribonuclease. II
- Amino Acids and Peptides. LIV. Application of 2-Adamantyl Derivatives as Protecting Groups to the Synthesis of Peptide Fragments Related to Sulfolobus solifataricus Ribonuclease. I
- Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae
- Galactose Depletion by Mortierella vinacea α-Galactosidase II Increases the Synergistic Interaction between Guar Gum and Xanthan Gum
- Nucleotide Sequence of α-Galactosidase cDNA from Mortierella vinacea
- In Vitro Sulfation of Glycoprotein with Sulfotransferase from Dictyosterium discoideum
- Growth Factors for a Primary Chick Muscle Cell Culture from Shochu Distillery By-products
- Role of Lys108 in the Enzymatic Activity of RNase Rh from Rhizopus niveus
- Effects of Corticosterone on Connectin Content and Protein Breakdown in Rat Skeletal Muscle
- Primary Structure, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from bacillus stearothermophilus
- Developmental Profile of Annexin IX and its Possible Role in Programmed Cell Death of the Bombyx mori Anterior Silk Gland(Developmental Biology)
- AN EXHAUSTIVE STUDY ON THE ECDYSONE-INDUCIBLE GENES IN THE SILKWORM BRAIN DURING METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EXPRESSION PROFILES OF ECDYSONE RECEPTOR ISOFORMS AND EARLY GENES ASSOCIATE THE PUPAL COMMITMENT IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- ECDYSTEROID CONTROLS PUPAL COMMITMENT AND CELL CYCLE IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HORMONAL CONTROL OF THE CELL CYCLE IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI
- Hormonal control of pupal commitment of wing imaginal disc in Bombyx mori(Endocrinology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- Isolation and Identification of 2-Phenylethyl Disaccharide Glycosides and Mono Glycosides from Rose Flowers, and Their Potential Role in Scent Formation
- OJ-075 Characteristics and Significance of Immediate Recurrence of Atrial Fibrillation after Catheter Ablation : A Close Relation to the Inflammatory Process(OJ13,Arrhythmia, Others (Clinical/Diagnosis/Treatment) 1 (A),Oral Presentation (Japanese),The 73r
- PE-495 Effects of Pulmonary Vein Isolation to Cardiac Function of Elderly Patients with Atrial Fibrillation(Arrhythmia, therapy(15)(A),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- On a Salmon (Onchorhynchus keta) Liver RNase, Belonging to RNase T2 Family : Primary Structure and Some Properties
- Effect of Replacing the Aspartic Acid / Glutamic Acid Residues of Bullfrog Sialic Acid Binding Lectin with Asparagine / Glutamine and Arginine on the Inhibition of Cell Proliferation in Murine Leukemia P388 Cells
- Triiodothyronine but Not Thyroxine Accelerates Myofibrillar Proteolysis via ATP Production in Cultured Muscle Cells(Food & Nutrition Sience)
- Effects of Corticosterone on Ca^ Uptake and Myofibrillar Disassembly in Primary Muscle Cell Culture(Biochemistry & Molecular Biology)
- Role of Ca^ in corticosterone-induced muscle growth retardation
- Effects of the Thyroid Hormone on Differentiation, Growth, and Proteolysis in Cultured Muscle Cells during Serum Deprivation
- Dose Dependent Effects of Corticosterone of Proliferation and Differentiation in Chick Myoblasts
- Effects of Thyroid Hormones on Myofibrillar Proteolysis and Activities of Calpain, Proteasome, and Cathepsin in Primary Cultured Chick Muscle Cells
- An Established Hybridoma Clone Producing a Monoclonal Antibody against Vibrio anguillarum
- Surgical Treatment for Acute Ischemia Caused by Bilateral Popliteal Artery Entrapment Syndrome in an Elderly Patient : Report of a Case
- A New Type of RNase T2 Ribonuclease in Two Basidiomycetes Fungi, Lentinus edodes and Irpex lacteus(Biochemistry & Molecular Biology)
- Evaluation of Esophageal Peristalsis in Patients with Esophageal Tumors : Initial Experience with Cine MR Imaging
- An Abdominal Aortic Rupture Due to Seatbelt Blunt Injury : Report of a Case
- Large Perigraft Seroma After Aortoiliac Bypass with an Expanded Polytetrafluoroethylene Graft : Report of a Case
- Infrainguinal Bypass Surgery for Chronic Arterial Occlusive Disease Associated with Essential Thrombocythemia : Report of a Case
- Traumatic Cardiac Herniation Diagnosed by Echocardiography and Chest CT Scanning : Report of a Case
- Autoproteolytic Processing of Aspartic Proteinase from Sunflower Seeds
- Purification and Characterization of Aspartic Proteinase from Sunflower Seeds
- PURIFICATION OF THE ASPARTIC PROTEINASE FROM SUNFLOWER SEEDS
- Minimal deviation mucinous adenocarcinoma ('adenoma malignum') of the uterine corpus
- Current status of body MR imaging : fast MR imaging and diffusion-weighted imaging
- Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
- Short-Term Prognosis of Adaptive Servo-Ventilation Therapy in Patients With Heart Failure
- Schwannoma of the Brachial Plexus Presenting as an Enlarging Cystic Mass : Report of a Case
- On the Multiplication of Dislocation by the Cathodic Charging of Hydrogen into Electrolytic Iron and Chromium Steels
- Effect of Short Duration-Adaptive Servo-Ventilation Therapy on Cardiac Function in Patients With Heart Failure
- A Case of CRTD with Inappropriate Ventricular Pacing Due to T Wave Over-Sensing
- A Case Showing Close Relationship between the Pulse Instability and the Respiratory Instability Using Polysomnography Analysis