Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With αMyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
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概要
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Background: The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. Methods and Results: We generated transgenic mice expressing mCherry-LC3 under αMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation. Conclusions: This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes.
著者
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Koyama Takashi
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
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Nobori Kiyoshi
Department Of Cardiovascular Medicine Akita University Graduate School Of Medicine
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Iino Kenji
Department Of Cardiology Akita Redcross Hospital
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TERADA Mai
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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KAKIZAKI Manabu
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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TAKAHASHI Yoichiro
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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TERATA Yutaka
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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Kakizaki Manabu
Department Of Cardiology Akita City General Hospital
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Munehisa Yoshiko
Department Of Cardiovascular And Respiratory Medicine Akita University Graduate School Of Medicine
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Hasegawa Hitoshi
Department Of Bioregulatory Medicine Ehime University Graduate School Of Medicine
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Takahashi Yoichiro
Department Of Cardiovascular Medicine Akita University Graduate School Of Medicine
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Terata Yutaka
Department Of Cardiovascular Medicine Akita University Graduate School Of Medicine
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Ito Hiroshi
Department Neurosurgery Tokyo Medical College
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Watanabe Hiroyuki
Department Of Agricultural Chemistry Ibaraki University
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Kosaka Toshimitsu
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
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Ishida Masaru
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine, Japan
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Ohba Takayoshi
Department of Cardiology, Cardiovascular Center, Nippon Medical School Chiba-Hokusoh Hospital
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Kosaka Toshimitsu
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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Hasegawa Hitoshi
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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Koyama Takashi
Department of Cardiology, Akita University School of Medicine
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Ishida Masaru
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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Iino Kenji
Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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