Primary Structure of a Base Non-specific and Adenylic Acid Preferential Ribonuclease from the Fruit Bodies of Lentinus edodes

概要

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The complete primary structure of a base non-specific and adenylic acid preferential RNase (RNase Le_2) from the fruit bodies of Lentinus edodes was analyzed. The sequence was mostly determined by analysis of the peptides generated by V_8 protease digestion and BrCN cleavage (including α-chymotryptic, and V_8 protease digest of BrCN fragments). It consists of 239 amino acid residues. The molecular weight is 25831. The location of 10 half cystine residues were almost superimposable on those of known fungal RNases of the RNase T_2 family. The sequence homologies between RNase Le_2 and four known fungal RNases of the RNase T_2 family, RNase T_2,RNase M, RNase Trv, and RNase Rh, are 102,103,109,and 74,respectively. The homologous sequences are concentrated around the three histidines, which are supposed to form the active site of RNase T_2 family RNases.
社団法人日本農芸化学会の論文
1992-12-23