Identification of the Glutamyl and Histidyl Residues Involved in the Active Site of a Minor Ribonuclease from Aspergillus saitoi(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
A glutamyl and two histidyl residues that are suspected to be part of the active site of a base non-specific, guanine-preferential ribonuclease from Aspergillus saitoi (RNase Ms) were located. RNase Ms modified by radioactive iodoacetate was digested by α-chymotrypsin, then separated by paper chromatography and paper electrophoresis. The amino acid composition of the radioactive peptide indicated that the site of the modification was Glu_<57>. A simple procedure to separate three histidyl-residue-containing peptides from RNase Ms was established. The procedure was used on the photooxidized RNase Ms, and the photooxidized histidyl residues were found to be His_<39> and His_<91>.
- 社団法人日本農芸化学会の論文
- 1986-05-23
著者
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Watanabe Hideaki
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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Watanabe H
Univ. Tokyo Tokyo Jpn
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Watanabe Hideaki
Department Of Microbiology Hoshi College Of Pharmacy
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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ENOMOTO Kumi
Department of Microbiology, Hoshi College of Pharmacy
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Watanabe Hideaki
Department Of Dermatology Showa University School Of Medicine
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Enomoto Kumi
Department Of Microbiology Hoshi College Of Pharmacy
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Watanabe Hideaki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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Watanabe Hideaki
Department of Applied Biological Chemistry, The University of Tokyo
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WATANABE HIDEAKI
Department of Anesthesiology, Kurume University School of Medicine
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