Identification of the Glutamyl and Histidyl Residues Involved in the Active Site of a Minor Ribonuclease from Aspergillus saitoi(Biological Chemistry)

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概要

• 論文の詳細を見る

• A glutamyl and two histidyl residues that are suspected to be part of the active site of a base non-specific, guanine-preferential ribonuclease from Aspergillus saitoi (RNase Ms) were located. RNase Ms modified by radioactive iodoacetate was digested by α-chymotrypsin, then separated by paper chromatography and paper electrophoresis. The amino acid composition of the radioactive peptide indicated that the site of the modification was Glu_<57>. A simple procedure to separate three histidyl-residue-containing peptides from RNase Ms was established. The procedure was used on the photooxidized RNase Ms, and the photooxidized histidyl residues were found to be His_<39> and His_<91>.

• 社団法人日本農芸化学会の論文
• 1986-05-23

著者

• Irie Masachika

  Department of Microbiology, Hoshi College of Pharmacy

• Watanabe Hideaki

  Department of Microbiology, Hoshi College of Pharmacy

• Ohgi Kazuko

  Department of Microbiology, Hoshi College of Pharmacy

• Ohgi K

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Ohgi Kazuko

  Department Of Microbiology Hoshi College Of Pharmacy

• Irie Masachika

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Irie Masachika

  Department Of Microbiology Hoshi College Of Pharmacy

• Watanabe H

  Univ. Tokyo Tokyo Jpn

• Watanabe Hideaki

  Department Of Microbiology Hoshi College Of Pharmacy

• Irie M

  Hoshi Coll. Pharmacy Tokyo Jpn

• ENOMOTO Kumi

  Department of Microbiology, Hoshi College of Pharmacy

• Watanabe Hideaki

  Department Of Dermatology Showa University School Of Medicine

• Enomoto Kumi

  Department Of Microbiology Hoshi College Of Pharmacy

• Watanabe Hideaki

  Department Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Ohgi Kazuko

  Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University

• Ohgi K

  Department Of Microbiology Hoshi College Of Pharmacy

• Watanabe Hideaki

  Department of Applied Biological Chemistry, The University of Tokyo

• WATANABE HIDEAKI

  Department of Anesthesiology, Kurume University School of Medicine

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