Purification and Properties of a Novel Surface-active Agent- and Alkaline-resistant Protease from Bacillus sp. Y(Biological Chemistry)
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概要
- 論文の詳細を見る
In the course of a search an alkaline stable protease for industrial use, an alkaline protease (protease BYA) was isolated from an alkalophilic Bacillus sp. Y, and its properties were characterized. Its optimum pH was pH 10.0-12.5, when casein was used as a substrate. In addition to the stability of protease BYA at pH 6.5-13.0, it was also very stable towards various surface-active agents, such as sodium dodecyl sulfate and sodium linear alkylbenzene sulfonate. Protease BYA was most active at 70℃. The isoelectric point (pl) of protease BYA was about 10.1. Protease BYA was characterized as a serine protease because of its sensitivity to phenylmethanesulfonyl fluoride and diiopropyl fluorophosphate. The protease seems to be related to proteases of the subtilisin family, such as subtilisin BPN', subtilisin Carlsberg, and No. 221 protease.
- 社団法人日本農芸化学会の論文
- 1991-09-23
著者
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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Oba Kenkichi
Research & Technology Headquarters Lion Corporation
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Iwama M
Department Of Microbiology Faculty Of Pharmaceutical Sciences Ohu-university
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Kudo Toshihiro
Biological Science Laboratories Lion Corporation
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SHIMOGAKI Hisao
Pharmaceutical Research Laboratories No.1, Pharmaceutical Products Division, Lion Corporation
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OHDERA Motoyasu
Biological Science Research Center, Lion Corporation
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SHIMOGAKI Hisao
Biological Science Laboratories, Lion Corporation
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TAKEUCHI Keiji
Biological Science Laboratories, Lion Corporation
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OHBA Kenkichi
Biological Science Laboratories, Lion Corporation
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Shimogaki Hisao
Pharmaceutical Research Laboratories No.1 Pharmaceutical Products Division Lion Corporation
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Ohdera Motoyasu
Biological Science Research Center Lion Corporation
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Takeuchi K
Yamaguchi Univ. School Of Medicine Yamaguchi Jpn
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Takeuchi Keiji
Biological Science Laboratories Of The Lion Corporation
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Ohdera Motoyasu
Life Sci. Res. Laboratories Lion Corp.
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