Molecular Cloning of Lipase Genes from Alcaligenes denitrificans and Their Expression in Escherichia coli
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概要
- 論文の詳細を見る
The lipase genes of Alcaligenes denitrificans TK7 and Pseudomonas aeruginosa OL1 were transferred to Escherichia coli JA2211 using RP4 : : Mu plasmid. Recombinant clones carrying the foreign lipase (lip) genes had 80 to 115% of the lipase activity of the donor strains of the lip genes. Cloning of the lip genes of A. denitrificans TK7 onto pBR322 revealed two Lip^+ recombinant plasmids harboring 15.7 Kb and 4.9 Kb foreign DNA segments, which were designated pYU1 and pYU2,respectively. That these two lip genes originated from A. denitrificans TK7 was demonstrated by Southern blot analysis. The lip gene of pYU1 was localized within a 1.1 Kb BglII-SalI segment and the lip gene of pYU2 was localized in a 0.8 Kb Hpa I-Pst I segment. E. coli harboring one of these derivatives produced 16 to 20 times more lipase than did A. denitrificans TK7.
- 社団法人日本生物工学会の論文
- 1986-10-25
著者
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Toh‐e A
Univ. Tokyo Tokyo Jpn
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Toh-e Akio
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Toh-e Akio
Department Of Fermentation Technology Hiroshima Universily
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TAKEUCHI Keiji
Biological Science Laboratories, Lion Corporation
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ODERA MOTOYASU
Biological Science Laboratories of the Lion Corporation
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Takeuchi Keiji
Biological Science Laboratories Of The Lion Corporation
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Toh-e Akio
Department of Biological Science, Graduate School of Science, University of Tokyo
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