Ribosomal DNA Plasmid Isolated from Zygosaccharomyces bailii and Its Use for Constructing Yeast Vectors Effective for Intergeneric Gene Transfer(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A part of the rDNA plasmid of Zygosaccharomyces bailii IFO 0488 has been cloned. The cloned sequence contained 18S, 25S and probably 5S rRNA sequences. The cloned rDNA of Z. bailii hybridized with rRNAs of Saccharomyces cerevisiae. On using this rDNA sequence as a probe, we found that every sample of closed circular DNA from yeasts tested contained this kind of DNA plasmid. The rDNA unit from Z. bailii carried an ARS that functioned in S. cerevisiae and Z. rouxii. However, the sequence required for the ARS function differed depending on the host. When a vector based on the rDNA of Z. bailii was introduced into S. cerevisiae, it was integrated predominantly into the RDNl locus. These facts imply that the rDNA plasmid is practically useful for contructing vectors suitable for intergeneric gene transfer among yeasts.
- 社団法人日本農芸化学会の論文
- 1986-06-23
著者
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Toh-e Akio
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Toh-e Akio
Department Of Fermentation Technology Hiroshima University
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OHISHI KAZUHIKO
Department of Molecular Pharmacology, Meiji College of Pharmacy
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Ohishi K
Okayama Univ. Tsushima Jpn
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Ohishi Kazuhiko
Department Of Molecular Pharmacology Meiji College Of Pharmacy
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SUGIHARA Kohji
Department of Fermentation Technology, Hiroshima University
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Sugihara Kohji
Department Of Fermentation Technology Hiroshima University
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Toh-e Akio
Department of Biological Science, Graduate School of Science, University of Tokyo
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OHISHI Kazuhiko
Department of Fermentation Technology, Hiroshima University
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