Secretion of Aspergillus oryzae Alkaline Protease in an Osmophilic Yeast, Zygosaccharomyces rouxii(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
To produce Aspergillus oryzae alkaline pro tease (Alp) in an osmophilic yeast Zygosaccharomyces rouxii, we constructed an expression plasmid consisting of the Z. rouxii glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter, the prepro-Alp cDNA of A. oryzae, the whole sequence of Z. rouxii plasmid pSRl, and the G418 resistant gene. The resulting plasmid, when introduced into Z. rouxii cells, directed the secretion of a large amount (about 300 mg/l) of Alp into the culture medium. The N-terminus and specific activity of the enzyme were identical to those of A. oryzae Alp.
- 社団法人日本農芸化学会の論文
- 1990-10-23
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Ishida Y
Research Division The Green Cross Corporation
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Toh-e Akio
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Toh-e Akio
Department Of Biology Faculty Of Science The University Of Tokyo
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Nakano Eiichi
Research And Development Division Kikkoman Corporation
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Murakami Seiji
Research And Development Division Kikkoman Corporation
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Aoki Makoto
Department Of Industrial Chemistry Faculty Of Engineering University Of Tokyo
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Ishida Yutaka
Research Division The Green Cross Corporation
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MURAKAMI Kohji
Research Division, The Green Cross Corporation
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MASAKI Atsushi
Research Division, The Green Cross Corporation
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TATSUMI Hiroki
Research and Development Division, Kikkoman Corporation
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OGAWA Yoshihiro
Research and Development Division, Kikkoman Corporation
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MOTAI Hiroshi
Research and Development Division, Kikkoman Corporation
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KAWABE Haruhide
Research Division, The Green Cross Corporation
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ARIMURA Hirofumi
Research Division, The Green Cross Corporation
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Ogawa Yoshihiro
Research And Development Division Kikkoman Corporation
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Murakami S
Kikkoman Corp. Chiba Pref. Jpn
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Murakami K
Institute For Marine Resources And Environment National Institute Of Advanced Industrial Science And
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Motai Hiroshi
Research And Development Division Kikkoman Corporation
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Tatsumi Hiroki
Research And Development Division Kikkoman Corporation
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Tatsumi Hiroki
(present Address)food Biotechnology Section Research Division Kikkoman Corporation
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Masaki Atsushi
Research Division The Green Cross Corporation
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Masaki Atsushi
Department Of Applied Physics Univetsity Of Tokyo
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Masaki Atsushi
Research Center For Development Of Far-infrared Region Fukui University
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Masuhara A
Institute For Multidisciplinary Research For Advanced Materials Tohoku University
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Kawabe Haruhide
Research Division The Green Cross Corporation
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Arimura Hirofumi
Research And Development Division The Green Grass Corporation
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Toh-e Akio
Department of Biological Science, Graduate School of Science, University of Tokyo
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NAKANO Eiichi
Research and Development Division, Kikkoman Corporation
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