Detection ofγ-Polyglutamic Acid (γ-PGA) by SDS-PAGE
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概要
- 論文の詳細を見る
γ-Polyglutamic acid (γ-PGA) produced by Bacillus subtilis (natto) was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and basic dye staining. Using this method, the molecular weight ofγ-PGA was estimated at 275,000. This value was almost the same in all bacterial strains tested. As other applications of this SDS-PAGE method, degradation of γ-PGA by acid and heat treatment and a cross-linking reaction with carbodiimide and ethylenediamine were made visible in acrylamide gel. In the growth curve of the bacteria, γ-PGA production was detected in early stationary phase by SDS-PAGE.
- 社団法人日本農芸化学会の論文
- 1996-02-23
著者
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Ogawa Yuji
Department Of Applied Bioorganic Chemistry Gifu University
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KIKUCHI Mamoru
Research and Development Division, Kikkoman Corporation
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OGAWA Yoshihiro
Research and Development Division, Kikkoman Corporation
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MOTAI Hiroshi
Research and Development Division, Kikkoman Corporation
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YAMAGUCHI Fumio
Research & Development Division, Kikkoman Corporation
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Yamaguchi F
Kikkoman Corp. Chiba Jpn
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Ogawa Yoshihiro
Research And Development Division Kikkoman Corporation
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Motai Hiroshi
Research And Development Division Kikkoman Corporation
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Kikuchi Mamoru
Research And Development Division Of Kikkoman Corporation
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Kikuchi Mamoru
Research & Development Division Kikkoman Corporation
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YUASA Katsumi
Research and Development Division, Kikkoman Corporation
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Yuasa K
Nagoya Univ. Nagoya Jpn
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Yuasa Katsumi
Technical Research Laboratories Asahi Chemical Industry Co. Ltd.
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Yamaguchi F
Kinki Polytechnic Coll. Osaka Jpn
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Yuasa Katsumi
Research And Development Division Kikkoman Corporation
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