Growth Characterizations of a Human Monocytic Leukemia Cell Line in a Serum-Free Medium

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A clone, AH-01S, derived from a human monocytic leukemia cell line, THP-1,grew rapidly in a serum-free-medium containing insulin, transferrin, ethanolamine, and sodium selenite. In batch culture using the serum-free medium, the AH-01S cells proliferated at a specific growth rate (μ) of 0.30 to 0.50 (1/day) from a cell concentration of 1×10^4 cells/ml to 1.6×10^6 cells/ml, an increase of 160 times. A higher cell concentration of 0.45×10^7 cells/ml (cell volume ratio was 0.5%) was obtained in spinner flask culture using the serum-free medium. A mean specific growth rate of 0.50 (1/day) was also observed in a culture in a fully instrumented cell culture fermentor. However, μ decreased drastically after the cell concentration reached 1.5×10^6 cells/ml. Analyses of medium composition during cultivation revealed that under lower cell concentration, L-glutamine was the main carbon source while glucose was converted to lactate almost stoichiometrically, and that the production of lactate from glucose decreased at higher cell concentration. To obtain cultures of 1×10^9 cells, 1,200 to 1,300 mg of a carbon source (glucose) and 400 to 500 mg of amino acids were consumed during high cell concentration cultivation of the AH-01S cells in the serum-free medium.
社団法人日本生物工学会の論文
1986-04-25