Efficient Production of γ-Polyglutamic Acid by Bacillus subtilis (natto) in Jar Fermenters
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概要
- 論文の詳細を見る
The large scale fermentation of γ-polyglutamic acid (γ-PGA) by Bacillus subtilis (natto) was done using a 30-liter jar fermenter. A stable cultivation without foaming could be done with addition of 3% NaCl to the medium. The γ-PGA productivity became higher with increasing speed of agitation and amounts of glutamic acid added to the broth. Finally, we were able to obtain about 35 mg/ml of γ-PGA under the optimum conditions. The glutamic acid added to the medium was efficiently converted into γ-PGA in the stationary phase. To discover the role of L-glutamic acid added to the medium for γ-PGA biosynthesis by Bacillus subtilis (natto), the radioactivity incorporated into γ-PGA from ^<14>C-L-glutamic acid was measured. As a result, radioactive γ-PGA was detected in the medium. Then, the glutamic acid in the medium was transported into the cells and actually polymerized as the glutamic acid unit of γ-PGA.
- 社団法人日本農芸化学会の論文
- 1997-10-23
著者
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Ogawa Yuji
Department Of Applied Bioorganic Chemistry Gifu University
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OGAWA Yoshihiro
Research and Development Division, Kikkoman Corporation
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YAMAGUCHI Fumio
Research & Development Division, Kikkoman Corporation
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Yamaguchi F
Kikkoman Corp. Chiba Jpn
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Ogawa Yoshihiro
Research And Development Division Kikkoman Corporation
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TAHARA Yasutaka
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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YUASA Katsumi
Research and Development Division, Kikkoman Corporation
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Tahara Y
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tahara Yasutaka
Department Of Applied Biological Chemistry Faculty Of Aglriculture
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Yuasa K
Nagoya Univ. Nagoya Jpn
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Yuasa Katsumi
Technical Research Laboratories Asahi Chemical Industry Co. Ltd.
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Yamaguchi F
Kinki Polytechnic Coll. Osaka Jpn
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Yuasa Katsumi
Research And Development Division Kikkoman Corporation
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