Cloning and High Expression of Catalase Gene from Bacillus sp.TE124
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概要
- 論文の詳細を見る
An efficient expression system for producing catalase in Bacillus was developed. A catalase was purified from Bacillus sp.TE124 and the catalase gene was cloned by plaque hybridization with a probe constructed from the N-terminal amino acid sequence of the enzyme. The gene, containing an open reading frame of 1452 bp, was subcloned into pHY300PLK for self-cloning into the organism. As a result, the production of catalase increased 20-fold over that of the parent strain.
- 公益社団法人日本生物工学会の論文
- 2001-04-25
著者
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Ni Jinfeng
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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KAWAMURA YOSHIHISA
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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Tokuyama Shinji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tokuyama Shinji
Technology Department Laboratries Takeda Chemical Industries Ltd.
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Sogabe Atsushi
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Sogabe Atsushi
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd
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TAHARA Yasutaka
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
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Tahara Y
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tahara Yasutaka
Department Of Applied Biological Chemistry Faculty Of Aglriculture
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Kawamura Yoshihisa
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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