Difference in Transcription Levels of cap Genes for γ-Polyglutamic Acid Production between Bacillus subtilis IFO16449 and Marburg 168
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概要
- 論文の詳細を見る
In a strain carrying capB-lacZ fusion of Bacillus subtilis IFO16449, which produces a large amount of γ-polyglutamic acid (PGA), β-galactosidase activity was enhanced by about five times with the addition of L-glutamic acid. This increase was also confirmed by Northern blot analysis. On the other hand, the activity was not detected in a strain carrying capB-lacZ fusion of B. subtilis Marburg 168. However, when the cap genes (capBCA and ywtC) were fused to the IPTG-inducible spac promoter, B. subtilis Marburg 168 produced PGA. These results suggest that the inability of B. subtilis Marburg 168 to produce PGA is due to defective expression of the cap genes.
- 公益社団法人日本生物工学会の論文
- 2002-02-25
著者
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Tokuyama Shinji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tahara Yasutaka
Department Of Applied Biological Chemistry Faculty Of Aglriculture
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URUSHIBATA YUJI
Department of Applied Biological Chemistry Faculty of Agriculture, Shizuoka University
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Urushibata Yuji
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Tokuyama Shinji
Department of Applied Biological Chemistry Faculty of Agriculture, Shizuoka University
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Tahara Yasutaka
Department of Applied Biological Chemistry Faculty of Agriculture, Shizuoka University
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