Spontaneous Reactivation of Shiga Toxins in Escherichia coli O157:H7 Cells Caused by Transposon Excision
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概要
- 論文の詳細を見る
IS1203v is an insertion sequence which has been found in inactivated Shiga toxin 2 genes (stx2) of Escherichia coli O157:H7. Using PCR amplification, we detected the wild-type stx2 genes in colonies of E.coli O157:H7 which possessed stx2 genes inactivated by insertion of IS1203v. This suggests that IS1203v is excised from the inactivated stx2 genes in E.coli O157:H7. We isolated the cells possessing the wild-type stx2 genes, and confirmed Stx2 productivities by reversed passive latex agglutination. We also analyzed the frequency of the appearance of the Stx2-producing cells using a quantitative PCR method. As a result, the frequency was 3.00×10^<-6> with culturing for 24 h at 37℃, and this increased to 8.83×10^<-5> when E.coli O157:H7 possessing the inactivated stx2 genes was transformed by an expression plasmid harboring the IS1203v transposase. These results showed that some Stx2-nonproducing E.coli O157:H7 strains could be spontaneously changed into Stx2-producing cells.
- 社団法人日本生物工学会の論文
- 2001-08-25
著者
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Suzuki Rieko
Biology Division Kanagawa Prefectural Institute Of Public Health
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Suzuki Rieko
Department Of Bacteriology And Pathology Kanagawa Prefectural Public Health Laboratory
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KAWAMURA YOSHIHISA
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd.
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Kusumoto Masahiro
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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YAMAI Shiro
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
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Yamai S
Kanagawa Prefectural Inst. Public Health Kanagawa
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Yamai Shiro
Department Of Bacteriology And Pathology Kanagawa Prefectural Public Health Laboratory
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OKITSU TADAYUKI
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory
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NISHIYA YOSHIAKI
Tsuruga Institute of Biotechnology, Toyobo Co.Ltd
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Okitsu T
Regional Hygiene Inspection Division Odawara Branch Kanagawa Prefectural Institute Of Public Health
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Kawamura Y
National Food Research Inst. Tsukuba Jpn
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Kawamura Y
Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.
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Nishiya Yoshiaki
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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Kawamura Yoshihisa
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
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