Continuous Production of Phospholipase D by Streptomyces lydicus D-121 Immobilized with Cross-Linked Chitosan Beads

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概要

• 論文の詳細を見る

• Continuous production of phospholipase D (PLD) in an air-lift reactor system was investigated using growing cells of Streptomyces lydicus D-121 immobilized within cross-linked chitosan beads as porous supports. In a continuous culture with the immobilized growing cells, both the activity and productivity were highest at a dilution rate of 0.330 h^<-1> which was higher than the maximum specific growth rate of the free cells (0.247 h^<-1>). The highest PLD activity was obtained at pH 6.0-6.5. The average activity was 12.7 U/ml during 120-384 h in a continuous culture with immobilized cells, while the productivity of the continuous culture (4.19×10^3 U/l・h) was about 3.3-3.6-fold higher than that of the batch culture (1.16×10^3 u/l・h) and the repeated-batch culture in the reactor (1.27×10^3 U/l・h), and about 3-fold higher than that in the case of continuous culture with free cells (1.36×10^3 U/l・h).

• 社団法人日本生物工学会の論文
• 1996-04-25

著者

• YAMANE TSUNEO

  Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo

• Yamane T

  Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University:

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Yamane T

  Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago

• Yamane T

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Yabuki Takashi

  Protein Research Group Riken Genomic Sciences Center

• Kawamura Y

  National Food Research Inst. Tsukuba Jpn

• SHINONAGA MASA-AKI

  Institute of Research & Development, Fuji Spinning Co. Ltd.,

• KAWAMURA YOSHIHIDE

  Institute of Research & Development, Fuji Spinning Co. Ltd.,

• SHIMBO KIKUO

  Department of Food R & D, Honen Corporation

• Kawamura Y

  Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.

• Shinonaga M

  Institute Of Research & Development Fuji Spinning Co. Ltd.

• Yamane Tsuneo

  Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University

• Shimbo Kikuo

  Department Of Food R & D Honen Corporation

• Yamane Tsuneo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

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