Purification and Properties of a Thermostable Esterase of Bacillus stearothermophilus Produced by Recombinant Bacillus brevis
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概要
- 論文の詳細を見る
A thermostable carboxyl esterase of Bacillus stearothermophilus excreted by recombinant B. brevis was purified to homogeneity using column chromatographies on DEAE Bio-Gel A, Sephacryl S-200HR, and Mono Q. The purified enzyme had a molecular weight of 29,000. The optimum pH at 37℃ was 7.5. The esterase had higher activity toward triglycerides with short-chain fatty acids than with long-chain ones. The activity of esterase was completely or significantly inhibited by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, iodoacetamide, and p-chloromercuribenzoic acid, while it was slightly stimulated in the presence of 2-mercaptoethanol. This observation suggests that that the enzyme is a serine enzyme having a sulfhydryl group for expressing the enzyme activity. The activation energy for inactivation of the enzyme was estimated to be 131 kcal・mol^<-1>. The kinetic parameters of this enzyme with p-nitrophenyl butyrate were measured : K_m was 0.132 mM, and V_max was 4.8 μmol・(mg protein)^<-1>・min<-1>.
- 社団法人日本農芸化学会の論文
- 1992-02-23
著者
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Ueda Shigeharu
Research Institute for Microbial Diseases, Osaka University
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Ueda S
Research Institute For Microbial Diseases Osaka University
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Ohmiya Kunio
Laboratory of Applied Microbiology, Faculty of Bioresources, Mie University
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Ohmiya Kunio
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Ueda Shunsaku
Laboratory Of Applied Microbiology Department Of Bioproductive Science Faculty Of Agriculture Utsuno
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Tulin Escuadra
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Ueda Shunsaku
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Tulin Edgard
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Amaki Yusuke
Laboratory of Bioreaction Engineering, Department of Food Science and Technology, School of Agricult
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Amaki Yusuke
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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