Acquisition of Data from On-Line Laser Turbidimeter and Calulation of Some Kinetic Variables in Computer-Coupled Automated Fed-Batch Culture
スポンサーリンク
概要
- 論文の詳細を見る
Output signals of a commercially available on-line laser turbidimeter exhibit fluctuations due to air and/or CO_2 bubbles. A simple data processing algorithm and a personal computer software have been developed to smooth the noisy turbidity data acquired, and to utilize them for the on-line calculations of some kinetic variables involved in batch and fed-batch cultures of uniformly dispersed microorganisms. With this software, about 10^3 instantaneous turbidity data acquired over 55 s are averaged and convert it to dry cell concentration, X, every minute. Aslo, volume of the culture broth, V, is estimated from the averaged output data of weight loss of feed solution reservoir. W, using an electronic balance on which the reservoir is placed. Then, the computer software is used to perform linear regression analyses over the past 30 min of the total biomass, VX, the natural logarithm of the total biomass, ln(VX), and the weight loss, W, in order to calculate volumetric growth rate, d(VX)/dt, specific growth rate, μ [=dln(VX)/dt] and the rate of W, dW/dt, every minute in a fed-batch culture. The software used to perform the first-order regression analyses of VX, ln(VX) and W was applied to batch or fed-batch cultures of Escherichia coli on minimum synthetic or natural complex media. Sample determination coefficients of the three different variables (VX, ln(VX) and W) were close to unity, indicating that the calculations are accurate. Furthermore, growth yield Y_<x/s>, and specific substrate consumption rate, q_<sc>, were approximately estimated from the data, d(VX)/dt and dW/dt in a 'balanced' fed-batch culture of E. coli on the minimum synthetic medium where the computer-aided substrate-feeding system automatically matches well with the cell growth.
- 社団法人日本生物工学会の論文
- 1995-07-25
著者
-
YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
-
Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Bioagricultural Sciences Nagoya University:
-
Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
-
Yamane Tsuneo
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
-
Miyaoku Kohei
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
-
Yamane T
Laboratory Of Molecular Biotechnology Graduate School Of Biological & Agricultural Sciences Nago
-
Yamane T
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
-
Miyamoto Kohji
Laboratory Of Molecular Biotechnology Department Of Applied Biological Science School Of Agriculture
-
Yabuki Takashi
Protein Research Group Riken Genomic Sciences Center
-
Miyamoto K
Faculty Of Pharmaceutical Sciences Osaka University
-
KADOTANI YASUYUKI
Central Engineering & Construction Co. Ltd.
-
MISHIMA NAOTO
Laboratory of Molecular Biotechnology, Department of Applied Biological Science, School of Agricultu
-
KOMINAMI MINORU
Central Engineering & Construction Co. Ltd.
-
Mishima Naoto
Laboratory Of Molecular Biotechnology Department Of Applied Biological Science School Of Agriculture
-
Yamane Tsuneo
Dept. Of Food Science And Technology Faculty Of Agriculture Nagoya University
-
Kadotani Yasuyuki
Central Engineering & Construction Co. Ltd.
-
Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
関連論文
- Stabilization of Affinity-Tagged Recombinant Protein during/after Its Production in a Cell-Free System Using Wheat-Germ Extract
- Cellular Toxicity of Cadmium Ions and Their Detoxification by Heavy Metal-Specific Plant Peptides, Phytochelatins, Expressed in Mammalian Cells^1
- Removal of Hazardous Phenols by Microalgae under Photoautotrophic Conditions
- Gene Analysis and Enzymatic Properties of Thermostable β-Glycosidase from Pyrococcus kodakaraensis KODI
- In Vitro Construction and Screening of a Burkholderia cepacia Lipase Library Using Single-Molecule PCR and Cell-Free Protein Synthesis(METHODS)
- Single-Step Single-Molecule PCR or DNA with a Homo-Priming Sequence Using a Single Primer and Hot-Startable DNA Polymerase
- 755 Monitoring of lipase-catalyzed transesterification between eicosapentaenoic acid ethyl ester and tricaprylin by silver ion high performance liquid chromatography and high temperature gas chromatography
- Rapid Method for Detection and Detoxification of Heavy Metal Ions in Water Environments Using Phytochelatin
- Characteristics of Biological NOx Removal from Flue Gas in a Dunaliella tertiolecta Culture System
- P-405 SITE-SPECIFIC FUNCTIONALIZATION OF PROTEINS BY ORGANOPALLADIUM REACTIONS
- Mechanism of Adaptation and H_2 Photoproduction in a Marine Green Alga, Chlamydomonas sp. MGA 161
- Isolation and Characterization of a Unicellular Marine Green Alga Exhibiting High Activity in Dark Hydrogen Production(Microbiology & Fermentaion Industry)
- Improvements in the Cell-Free Production of Functional Antibodies Using Cell Extract from Protease-Deficient Escherichia coli Mutant (Methods)
- Improvement of Selective Removal of Heavy Metals in Cyanobacteria by NaOH Treatment(Environmental Biotechnology)
- Degradation of Carbendazim and 2, 4-Dichlorophenoxyacetic Acid by Immobilized Consortium on Loofa Sponge(ENVIRONMENTAL BIOTECHNOLOGY)
- S205 Microalgae : key bioresources for green technology
- 827 Biodegradation of agriculture toxic chemicals by microbial consortium
- Effect of Algal Extract on H_2 Production by a Photosynthetic Bacterium Rhodobium marinum A-501 : Analysis of Stimulating Effect Using a Kinetic Model(ENVIRONMENTAL BIOTECHNOLOGY)
- Improvement of Microalgal NOx Removal in Bubble Column and Airlift Reactors
- Selective Cadmium Removal from Hard Water Using NaOH-Treated Cells of the Cyanobacterium Tolypothrix tenuis
- Removal of Cadmium from Aqueous Solution by the Filamentous Cyanobacterium Tolypothrix tenuis
- Biological Elimination of Nitric Oxide and Carbon Dioxide from Flue Gas by Marine Microalga NOA-113 Cultivated in a Long Tubular Photobioreactor
- 722 Accumulation of Heavy Metals by Blue-Green Algae
- Photoproduction of Hydrogen from Raw Starch Using a Halophilic Bacterial Community
- Hydrogen Photoproduction from CO_2-Fixing Microalgal Biomass : Application of Halotolerant Photosynthetic Bacteria
- Hydrogen Photoproduction from CO_2-Fixing Microalgal Biomass : Application of Lactic Acid Fermentation by Lactobacillus amylovorus
- Environmentally friendly production of H_2 Incorporating microalgal CO_2 fixation
- Modulator-Mediated Synthesis of Active Lipase of Pseudomonas sp.109 by Escherichia coli Cell-Free Coupled Transcription/Translation System
- Biosynthetic Regulation of Phytochelatins, Heavy Metal-Binding Peptides
- H_2 Production from Algal Biomass by a Mixed Culture of Rhodobium marinum A-501 and Lactobacillus amylovorus
- Catharanthine Oxidation in Flavin Mononucleotide-Mediated Catharanthine-Vindoline Coupling Reaction for Synthesis of Dimeric Indole Alkaloids under Near-Ultraviolet Light
- Non-Enzymic Coupling of Vindoline and Catharanthine to Synthesize 3', 4'-Anhydrovinblastine under Irradiation with Near-Ultraviolet Light
- Accumulation of Translational Inhibitor durig Multi-Hour Cell-Free Protein Synthesis Reaction Using Rabbit Reticulocyte Lysate
- High Speed Polymerase Chain Reaction in Constant Flow
- 448 In vitro analysis of the roles of a disulfide bridge and a calcium binding site in Pseudomonas sp. KWI-56 lipase using PCR and Escherichia coli coupled transcription/translation
- Increasing Productivity by Removing Choline in Conversion of Phosphatidylcholine to Phosphatidylserine by Phospholipase D
- 573 Increased Productivity by Removing Choline in Conversion of Phosphatidyl-choline to Phosphatidylserine by Phospolipase D.
- 515 Increasing Phosphatidylcholine Content of Lecithin by Phospholipase D
- 226 Enzymatic Conversion of Phosphatidylcholine to Phosphatidylethanolamine by Phospholipase D from Different Sources
- 327 Continuous Ethanol Fermentation Using Flocculent Yeast Entrapped in Horizontal Parallel Flow Bioreactor System
- Phenomenological Background and Some Preliminary Trials of Automated Substrate Supply in pH-Stat Modal Fed-Batch Culture Using a Setpoint of High Limit
- L-Serine Production from Methanol and Glycine with an Immobilized Methylotroph
- Economic Significance of Increasing Cell Mass Concentration for Metabolite Production : A Theoretical Study
- Effect and Control of Glucose Feeding on Bacitracin Production by Fed-Batch Culture of Bacillus licheniformis
- Production of L-Serine from Methanol and Glycine by Resting Cells of a Methylotroph under Automatically Controlled Conditions
- Control of Carbon Source Supply and Dissolved Oxygen by Use of Carbon Dioxide Concentration of Exhaust Gas in Fed-Batch Culture
- Detection and Automatic Control of Ammonium Ion Concentration in Microbial Culture with an Ammonium Ion Selective Electrode
- 565 Charcteristics of Batch Culture of a Recombinant ___- ___- Producing a Foreign Esterase
- Comprison of Cell Productivities among Fed-Batch, Repeated Fed-Batch and Continuous Cultures at High Cell Concentration
- Inhibition and Inactivation of Lipase by Fat Peroxide in the Course of Batch and Continuous Glycerolyses of Fat by Lipase(Biological Chemistry)
- Selective Hydrolysis of Fish Oil by Lipase to Concentrate n-3 Polyunsaturated Fatty Acids(Biological Chemistry)
- Purification and Some Characteristics of Extracellular Lipase from Fusarium oxysporum f.sp.lini
- In Vitro Selection of DNA Binding Sites for Transcription Factor, PhaR, from Paracoccus denitrificans Using Genetic Library on Microbeads and Flow Cytometry(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Production and Characterization of Monoclonal Antibodies against Occluded Virion of Nuclear Polyhedrosis Virus from Bombyx mori(Lepidoptera:Bombycidae)
- Effects of the Hemolymph from Bombyx mori, Immunized with Escherichia coli, on the Proliferation of Plant Pathogenic Bacteria(Biological Chemistry)
- 1F14-1 Enzymatic preparation of enantiomerically pure 2,3-diacylglycerol
- 2J10-5 Direct separation of regio- and enantiomeric isomers of diacylglycerols by a tandem column high-performance liquid chromatography
- Expression Profiles of Polyhydroxyalkanoate Synthesis-Related Genes in Paracoccus denitrificans
- Cloning and in Vitro and in Vivo Expression of Plant Glutathione S-Transferase Zeta Class Genes(GENETICS,MOLECULAR BIOLOGY,AND GENE ENGINEERING)
- Reduction of Protein Degradation by Use of Protease-Deficient Mutants in Cell-Free Protein Synthesis System of Escherichia coli
- Dosage Effect of Minor Arginyl- and Isoleucy1-tRNAs on Protein Synthesis in an Escherichia coli In Vitro Coupled Transcription/Translation System
- Importance of Disulfide Bridge Formation on Folding of Phospholipase D from Streptomyces antibioticus
- Continuous Production of Phospholipase D by Streptomyces lydicus D-121 Immobilized with Cross-Linked Chitosan Beads
- Acquisition of Data from On-Line Laser Turbidimeter and Calulation of Some Kinetic Variables in Computer-Coupled Automated Fed-Batch Culture
- Extracellular Production of Phospholipase D of Streptomyces antibioticus Using Recombinat Escherichia coli
- Prolonged Cell-free Protein Synthesis in a Batch System Using Wheat Germ Extract
- Phosphatidylglycerol Synthesis by Phospholipase D in a Microporous Membrane Bioreactor : Studies on Enzymatic Conversion of Phospholipids (I)
- 107 Effective Extracellular Production of Bacillus stearothermophilus Esterase by pH-stat modal Fed-batch Culture of Recombinant Bacillus brevis
- An Increased Rate of Cell-free Protein Synthesis by Condensing Wheat-germ Extract with Ultrafiltration Membranes
- Immobilization of Yeast Cells with Cross-Linked Chitosan Beads
- Vindoline and Catharanthine Production in Multiple Shoot Cultures of Catharanthus Roseus
- Site-specific incorporation of 4-lodo-L-phenylalanine through opal suppression
- Stably Sustained Hydrogen Production with High Molar Yield through a Combination of a Marine Green Alga and a Photosynthetic Bacterium
- Oxidation of 3-Hydroxypiperidines with Iodosylbenzene in Water : Tandem Oxidative Grob Fragmentation-Cyclization Reaction
- Three Automated Feeding Strategies of Natural Complex Nutrients Utilizing On-Line Turbidity Values in Fed-Batch Culture : A Case Study on the Cultivation of a Marine Microorganism
- Interesterification of Fats and Oils by Immobilized Fungus at Constant Water Concentration
- Kinetic Studies on the Interesterification of Oils and Fats Using Dried Cells of Fungus
- Screening for Fungi That Have High Lipolytic and Acidolytic Activities in Biomass Support Particles
- Avoidance of Solidification of Sesame Oil at Low Temperature by Self-interesterification with Immobilized Lipase
- Purification and Characterization of Nicotinic Acid Dehydrogenase from Pseudomonas fluorescens TN5
- Microbial Production of 6-Hydroxynicotinic Acid, an Important Building Block for the Synthesis of Modern Insecticides
- Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells of Pseudomonas fluorescens TN5
- A Bacillus stearothermophilus Esterase Produced by a Recombinant Bacillus brevis Stabilized by Sulfhydryl Compounds
- 316. Purification and Characterization of a Thermostable Bacillus stearothermophilus Esterase Excreted from a Recombinant Bacillus brevis
- Purification and Properties of a Thermostable Esterase of Bacillus stearothermophilus Produced by Recombinant Bacillus brevis
- Replica Technique for Screening Poly(3-Hydroxyalkanoic Acid)-Producing Bacteria by Nile Blue Staining
- Stimulation of Dimeric Alkaloid Production by Near-Ultraviolet Light in Multiple Shoot Cultures of Catharanthus roseus
- 115 IMMOBILIZATION OF UNICELLULAR MARINE GREEN ALGA AND FILAMENTOUS BLUE-GREEN ALGA
- Some Characteristics of Continuous Glyceride Synthesis by Lipase in a Microporous Hydrophobic Membrane Bioreactor
- Continuous Hydrolysis of Olive Oil by Lipase in Microporous Hydrophobic Hollow Fiber Bioreactor