Production and Characterization of Monoclonal Antibodies against Occluded Virion of Nuclear Polyhedrosis Virus from Bombyx mori(Lepidoptera:Bombycidae)
スポンサーリンク
概要
- 論文の詳細を見る
To identify structural protein genes of the silkworm, Bombyx mori nuclear polyhedrosis virus (BmNPV), monoclonal antibodies (MAbs) were raised against the occluded virion (OV) and characterized. Out of 35 MAbs obtained, 10 belonged to immunoglobulin G (IgG) class. Two IgG class MAbs reacted with polyhedra. three of the other 8 IgG class MAbs, designated 7D2, 8D5 and EHA, reacted with 2 to 9 polypeptides of BmNPV-OV. The periodate oxidation of OV suggested that 2 MAbs of 7C8 and CD6 are specific for carbohydrates. The electronmicroscopic observation indicated that 7D2 and 7C8 are reactive to antigenic determinants, present on the nucleocapsid and envelope, respectively. These results suggest that 7D2 can be a good tool as a probe to screen genes for nucleocapsid structural proteins from a BmNPV genomic library.
- 日本応用動物昆虫学会の論文
- 1992-02-25
著者
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Miyaoku Kohei
Department Of Synthetic Chemistry And Biological Chemistry Graduate School Of Engineering Kyoto Univ
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TANIAI Kiyoko
Laboratory of Biological Defense, National Institute of Sincultural and Entomological Science
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YAMAKAWA Minoru
Laboratory of Biological Defense, National Institute of Sincultural and Entomological Science
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Yamakawa Minoru
Univ. Tsukuba Ibaraki Jpn
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Yamakawa M
National Inst. Agrobiological Sci. Ibaraki Jpn
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Yamakawa Minoru
Laboratory Of Biological Defenoe Department Of Insect Physiology And Behavior National Institute Of
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Miyamoto Kazuhisa
Laboratory Of Biological Defenoe Department Of Insect Physiology And Behavior National Institute Of
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Yamakawa Minoru
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Taniai K
National Inst. Agrobiological Sci. Ibaraki Jpn
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Miyamoto K
Faculty Of Pharmaceutical Sciences Osaka University
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NISHIMORI Kei
Physiological Active Substances Research Laboratory, Biological Products Research Division, National
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Taniai Kiyoko
Laboratory Of Biological Defense National Institute Of Sericultural And Entomological Science
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Nishimori Kei
Physiological Active Substances Research Laboratory Biological Products Research Division National I
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