Characteristics of Novel Insect Defensin-Based Membrane-Disrupting Trypanocidal Peptides
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概要
- 論文の詳細を見る
Synthetic D- and L-amino acid type cationic 9-mer peptides (all sequences were synthesized as D- or L-amino acids) derived from the active sites of insect defensins were tested for their ability to modify the growth of blood-stream form African trypanosomes in vitro. One of them, the D-type peptide A (RLYLRIGRR-NH2), irreversibly suppressed proliferation of the Trypanosoma brucei brucei GUTat3.1 parasite. The presence of negatively charged phosphatidylserine on the surface of the parasites was demonstrated, suggesting electrostatic interaction between the peptide and the phospholipids. Furthermore, this peptide was found to alter trypanosome membrane-potentials significantly, an effect apparently due to the removal of the parasite’s plasma membrane. The potential toxic effects of D-peptide A on mammalian cells was assessed using human brain microvascular endothelial cells. Only minor effects were found when the endothelial cells were exposed for 16 h to peptide concentrations of less than 200 μM. These findings suggest that insect defensin-based peptides represent a potentially new class of membrane-disrupting trypanocidal drugs.
- 社団法人 日本農芸化学会の論文
- 2009-07-23
著者
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KITANI Hiroshi
Transgenic Animal Research Center, National Institute of Agrobiological Sciences
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ISHIBASHI Jun
National Institute of Agrobiological Sciences
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YAMAKAWA Minoru
National Institute of Agrobiological Sciences
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Kitani Hiroshi
Transgenic Animal Research Center National Institute Of Agrobiological Sciences
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Ishibashi Jun
Innate Immunity Res. Unit National Inst. Of The Agrobiological Sciences
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Ishibashi Jun
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Iwasaki Takashi
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Yamakawa Minoru
Univ. Tsukuba Ibaraki Jpn
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Yamakawa M
National Inst. Agrobiological Sci. Ibaraki Jpn
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Yamakawa Minoru
Laboratory Of Biological Defenoe Department Of Insect Physiology And Behavior National Institute Of
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Yamakawa Minoru
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
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Yamakawa Minoru
Innate Immunity Laboratory National Institute Of Agrobiological Sciences
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Yamakawa Minoru
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Kubo Masanori
Department Of Clinical Research And Development Otsuka Pharmaceutical Co. Ltd.
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Ishibashi Jun
Innate Immunity Laboratory National Institute Of Agrobiological Science
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Iwasaki Takashi
Graduate School Of Life And Environmental Science University Of Tsukuba
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KUBO Masanori
Diagnostic Section, National Institute of Animal Health
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YOSHIYAMA Mikio
Innate Immunity Research Unit, National Institute of Agrobiological Sciences
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Kitani Hiroshi
Transgenic Animal Research Center National Institute Of The Agrobiological Sciences
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Kubo Masanori
Diagnostic Section National Institute Of Animal Health
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YAMAGE Mat
Innate Immunity Research Unit, National Institute of the Agrobiological Sciences
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GRAB Dennis
Department of Pediatrics, The Johns Hopkins School of Medicine
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Grab Dennis
Department Of Pediatrics The Johns Hopkins School Of Medicine
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Yamage Mat
Innate Immunity Research Unit National Institute Of The Agrobiological Sciences
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Yoshiyama Mikio
Innate Immunity Research Unit National Institute Of The Agrobiological Sciences
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Yoshiyama Mikio
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
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Kubo Masanori
Department of Applied Chemistry, University of Osaka Prefecture
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