DNA Vector-Based RNA Interference in Cell Lines Derived from Bombyx mori
スポンサーリンク
概要
- 論文の詳細を見る
Gene-knockdown technology using RNA interference (RNAi) is widely used to characterize gene functions in many organisms. In this study, we analyzed the conditions for employing DNA vector-based RNAi in silkworm cell lines using long-hairpin RNA-expressing plasmid DNA. We found that NIAS-Bm-oyanagi2 was the most effective cell line for RNAi. Expression of long-hairpin RNA containing an approximately 500 base-pair stem region suppressed expression of a reporter target gene by more than 99% in this cell line. Furthermore, the loop sequence of hairpin RNA was not as important to RNAi efficiency as previously observed in Drosophila melanogaster. DNA vector-based RNAi also induced significant suppression of endogenous clathrin in NIAS-Bm-oyanagi2. Luciferase activity from recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) containing luciferase in the clathrin-knockdown cells was significantly less than in the control cells, suggesting that clathrin is indispensable for the entry of BmNPV into silkworm cell lines.
- 社団法人 日本農芸化学会の論文
- 2009-09-23
著者
-
ISHIBASHI Jun
National Institute of Agrobiological Sciences
-
YAMAKAWA Minoru
National Institute of Agrobiological Sciences
-
Ishibashi Jun
Innate Immunity Res. Unit National Inst. Of The Agrobiological Sciences
-
Ishibashi Jun
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Yamakawa Minoru
Univ. Tsukuba Ibaraki Jpn
-
Yamakawa M
National Inst. Agrobiological Sci. Ibaraki Jpn
-
Yamakawa Minoru
Laboratory Of Biological Defenoe Department Of Insect Physiology And Behavior National Institute Of
-
Yamakawa Minoru
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
-
Yamakawa Minoru
Innate Immunity Laboratory National Institute Of Agrobiological Sciences
-
SAGISAKA Aki
Innate Immunity Research Unit, National Institute of Agrobiological Science
-
Sagisaka Aki
Innate Immunity Research Unit National Institute Of Agrobiological Science
-
Tanaka Hiromasa
Faculty Of Agriculture Iwate University
-
Yamakawa Minoru
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
-
TANAKA Hiromitsu
Innate Immunity Research Unit, National Institute of Agrobiological Sciences
-
FUJITA Kosuke
Innate Immunity Research Unit, National Institute of Agrobiological Sciences
-
IMANISHI Shigeo
Genebank, National Institute of Agrobiological Sciences
-
Ishibashi Jun
Innate Immunity Laboratory National Institute Of Agrobiological Science
-
Tanaka H
Innate Immunity Laboratory National Institute Of Agrobiological Science
-
Fujita Kosuke
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
-
Tomimoto Kazuya
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
-
Imanishi Shigeo
Genebank National Institute Of Agrobiological Sciences
-
Sagisaka Aki
Innate Immunity Research Unit National Institute Of Agrobiological Sciences
-
Tanaka Hiromitsu
Innate Immunity Laboratory National Institute Of Agrobiological Sciences
関連論文
- Chitin Binding Activity of Oryctes rhinoceros Antimicrobial Peptides
- The Solution Structure of Diapause-Specific Peptide
- Signal Transudation for Cecropin B Gene Expression in Adherent Hemocytes of the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
- Isolation and Identification of Adipokinetic Hormone of the Silkworm, Bombyx mori
- Antibacterial and Anticancer Activity of Diastereomeric 9-mer Peptides Derived from Beetle Defensins
- In vitro Activity of Diastereomeric Antimicrobial Peptides Alone and in Combination with Antibiotics against Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa
- A Short Peptide Synthesized on the Basis of Coleoptericin A from Allomyrina dichotoma Shows Similar Anti-Escherichia coli Action
- B226 Regulation of defensin antimicrobial genes in the soft tick Ornithodoros moubata (Acari : Argasidae)
- B15 Spectrum and killing mechanisms of defensin antimicrobial peptides in Ornithodoros moubata (Acari : Argasidae)
- B07 Defensin gene regulation in Ornithodoros moubata(Acari : Argasidae)(The 59th Annual Meeting of the Japan Society of Medical Entomology and Zoology)
- Baculovirus-Mediated Production and Antifungal Activity of a Diapause-Specific Peptide, Diapausin, of the Adult Leaf Beetle, Gastrophysa atrocyanea : Coleoptera : Chrysomelidae
- A specific peptide produced during adult diapause of the leaf beetle, Gastrophysa atrocyanea Motschulsky (Coleoptera : Chrysomelidae)
- Multiple Functions of Short Synthetic Enantiomeric Peptides Based on Beetle Defensins
- Effects of Antimicrobial Peptides Derived from the Beetle Allomyrina dichotoma Defensin on Mouse Peritoneal Macrophages Stimulated with Lipopolysaccharide
- Analysis of the 5'-upstream regulatory region of a gene encoding coleoptericin, an antibacterial protein from the beetle, Allomyrina dichotoma (Coleoptera: Scarabaeidae)
- cDNA cloning and gene expression of defensin, an antibacterial peptide from a beetle, Allomyrina dichotoma (Coleoptera : Scarabaeidae)
- Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity
- A Double Chaperone Function of the sHsp Genes against Heat-Based Environmental Adversity in the Soil-Dwelling Leaf Beetles
- H305 FUNCTIONAL ANALYSIS OF AN ADULT DIAPAUSE-ASSOCIATED PROTEIN FROM THE LEAF BEETLE GASTROPHYSA ATROCYANEA
- NMR structure analysis of ORYCIN from the coconut rhinoceros beetle, Oryctes rhinoceros, led to its functional characterization as a novel serine protease inhibitor
- Multi-target of enantiomeric 9-mer peptides derived from beetle defensins
- Functional characterization of a cactus homolog from the silkworm Bombyx mori
- Gene Expression of a Novel Defensin Antimicrobial Peptide in the Silkworm, Bombyx mori
- Molecular Cloning and Characterization of Histone H2A.Z Gene of the Silkworm, Bombyx mori
- Effect of nucleopolyhedrovirus infection on antibacterial activity and antibacterial peptide production in larvae of the silkworm, Bombyx mori(Lepidoptera: Bombycidae)
- Expression of Antimicrobial Peptide Genes Encoding Enbocin and Gloverin Isoforms in the Silkworm, Bombyx mori
- Regulation of Gene Expression of Attacin, an Antibacterial Protein in the Silkworm, Bombyx mori
- Spontaneous Synthesis of an Antibacterial Peptide Linked to Ecdysis in Lepidopteran Insects
- Inheritance and Stability of Resistance to Bacillus thuringiensis Formulations of the Diamondback Moth, Plutella xylostella(LINNAEUS)(Lepidoptera:Yponomeutidae)
- Partial nucleotide sequence of a cDNA encoding 18S ribosomal RNA from the coconut rhinoceros beetle, Oryctes rhinoceros Linnaeus
- Development of a Colorimetric Assay for Determining the Amount of H_2O_2 Generated in Tobacco Cells in Response to Elicitors and Its Application to Study of the Structure-Activity Relationship of Flagellin-Derived Peptides
- Production and Characterization of Monoclonal Antibodies against Occluded Virion of Nuclear Polyhedrosis Virus from Bombyx mori(Lepidoptera:Bombycidae)
- Effects of the Hemolymph from Bombyx mori, Immunized with Escherichia coli, on the Proliferation of Plant Pathogenic Bacteria(Biological Chemistry)
- Effect of a Synthetic Peptide Designed on the Basis of the Active Site of Insect Defensins on the Proliferation of Methicillin-resistant Staphylococcus aureus under the Conditions for External Application
- The brain neurosecretory cells of the moth Samia cynthia ricini: Immunohistochemical localization and developmental changes of the Samia homologues of the Bombyx prothoracicotropic hormone and bombyxin
- Deamidation of N-terminal Asparagine
- Interactions of an Antimicrobial Peptide Moricin with Lipid Bilayers
- Synthetic Oligopeptides Derived from Insect Defensin Have Antimicrobial Activity against Antibiotic-Resistant Bacteria and Dermatophytic Fungi
- Secondary Structure and Thermo-Stability of Oryctin
- Assignment of Disulfide Bond Location in Oryctin
- Cooperative Antibacterial Relationship between Lebocin and Cecropin D, Antibacterial Peptides Isolated from the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)
- Expression of a cysteine proteinase inhibitor gene in silkworm Bombyx mori following Trypanosoma brucei brucei challenge
- Effect of Moricin, a Novel Antibacterial Peptide of Bombyx mori (Lepidoptera: Bombycidae), on the Growth of Methicillin-Resistant Staphylococcus aureus (MRSA)
- Expression of antibacterial protein genes in ligated larvae of the silkworm, Bombyx mori
- Microarray Analysis of Gene Expression in MC3T3-E1 after Specific Far-infrared Radiation(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- Functional Characterization of a Cactus Homolog from the Silkworm Bombyx mori
- DNA Vector-Based RNA Interference in Cell Lines Derived from Bombyx mori
- Characteristics of Novel Insect Defensin-Based Membrane-Disrupting Trypanocidal Peptides
- Multiple Functions of Short Synthetic Enantiomeric Peptides Based on Beetle Defensins
- Gene Expression of a Novel Defensin Antimicrobial Peptide in the Silkworm, Bombyx mori
- Forming mechanism of extracellular matrix containing periodontal ligament-like collagen fiber and its bone inducing activity(15thAnnual Meeting of the Society for Hard Tissue Regenerative Biology,Session 2)
- TWO CASES OF ACUTE LEAD POISONING ON A KOREAN SHIP
- Correlation of Differential Expression of Silkworm Antimicrobial Peptide Genes with Different Amounts of Rel Family Proteins and Their Gene Transcriptional Activity
- Structure-based Functional Characterization of Oryctin, a Hemolymph Protein from the Coconut Rhinoceros Beetle, Oryctes Rhinoceros, As a Novel Serine Protease Inhibitor
- Anti-Angiogenesis Activities of Novel Peptide Complexes : Mitochondria-Disruptive 9mer Peptides Conjugated with the Integrin alpha V beta 3-Homing Cyclic RGD Motif
- Anti-Angiogenesis Activities of Novel Peptide Complexes : Mitochondria-Disruptive 9mer Peptides Conjugated with the Integrin alpha V beta 3-Homing Cyclic RGD Motif