A specific peptide produced during adult diapause of the leaf beetle, Gastrophysa atrocyanea Motschulsky (Coleoptera : Chrysomelidae)
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概要
- 論文の詳細を見る
In the adult stage of the leaf beetle, Gastrophysa atrocyanea, a diapause-specific peptide appeared at diapause initiation and disappeared after overwintering. Continuous JHA applications induced the diapause breakdown which caused the peptide to decline. To isolate the peptide, we used disk preparative SDS-PAGE, gel filtration HPLC and RP-HPLC. The apparent molecular mass of the peptide was 7.9 kDa with SDS-PAGE and 4466.3 Da by mass spectrometry. The first 36 the N-terminal amino acid sequence of the peptide showed : AVRIGPXDQVXPRIVPERHEXXRAHGRSGYAYXSGG. Antiserum prepared for the peptide was used for western blotting and immunohistochemistry. The results suggested that it is synthesized in the fat body and released into the hemolymph.
- 日本応用動物昆虫学会の論文
- 1998-11-25
著者
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SUZUKI Koichi
Department of Pediatrics, Hirosaki University School of Medicine
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Yamashita Tetsuro
Department Of Chemical Engineering Faculty Of Engineering Iwate University
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Suzuki Koichi
Faculty of Agriculture, Iwate University
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Sato K
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Yamashita Tetsuro
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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TANAKA Hiromasa
Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
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Tanaka Hiromasa
Faculty Of Agriculture Iwate University
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Tanaka Hiromasa
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Sato K
Tokyo University Of Agriculture And Technology
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Suzuki Koichi
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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SATO Kenji
Iwate Biotechnology Research Center
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Sudo Chie
Department of Bioscience and Technology, Faculty of Agriculture, lwate University
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An Ying
Department of Bioscience and Technology, Faculty of Agriculture, lwate University
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Kurihara Morihisa
Fuji University
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Sudo Chie
Department Of Bioscience And Technology Faculty Of Agriculture Lwate University
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Suzuki K
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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An Y
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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An Ying
Department Of Agro-bioscience Faculty Of Agriculture Iwate University
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Yamashita Tetsuro
Faculty Of Agriculture Iwate University
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Suzuki Koichi
Faculty Of Agriculture Iwate University
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