Immobilization of Yeast Cells with Cross-Linked Chitosan Beads

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概要

• 論文の詳細を見る

• Ethanol-producing yeast cells were entrapped with cross-linked chitosan beads (CCB). In repeated batch culture, the number of yeast cells immobilized with CCB finaly reached 1.2 × 10^<10> cells/ml-beads. While this result was almost the same as with calcium alginate, the ethanol concentration produced with CCB was about 1.5 times higher than that with calcium alginate. The most suitable CCB pore size to immobilize yeast cells was about 10 μm, while to produce ethanol it was approximately 15 μm. CCB modified chamically with the trimethylammonium group had a high capacity to immobilize yeast cells, but the ethanol concentration produced was almost the same as with unmodified CCB. CCB as a carrier had higher yeast cell immobilizing and ethanol production capacities than the commercial carriers SIRAN and FIBERM. In continuous culture, ethanol was produced for thirty days at least at an average concentration of 51g/l when the dilution rate was 0.1h^<-1>.

• 社団法人日本生物工学会の論文
• 1992-08-25

著者

• YAMANE TSUNEO

  Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo

• Yamane Tsuneo

  Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult

• Yamane Tsuneo

  Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur

• Kawamura Y

  National Food Research Inst. Tsukuba Jpn

• SHINONAGA MASA-AKI

  Institute of Research & Development, Fuji Spinning Co. Ltd.,

• KAWAMURA YOSHIHIDE

  Institute of Research & Development, Fuji Spinning Co. Ltd.,

• Kawamura Y

  Tsuruga Institute Of Biotecnology Tokyo Co. Ltd.

• Shinonaga M

  Institute Of Research & Development Fuji Spinning Co. Ltd.

• Yamane Tsuneo

  Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University

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