Production of L-Serine from Methanol and Glycine by Resting Cells of a Methylotroph under Automatically Controlled Conditions
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概要
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Serine production from methanol and glycine was tried using frozen-thawed resting cells of a methylotroph, Protomonas extorquens NR-1 under multi-variable controlled conditions. The conditions for L-serine formation were optimized at 30℃. The production of L-serine in 0.4% CaCl_2 solution (initial pH 8.2) was the same as in 0.1 M Tris-HCl buffer (initial pH 8.3). Increasing the initial glycine concentration promoted L-serine formation. A high aeration rate decreased L-serine production. The optimum concentrations of dissolved oxygen and methanol were 0.5 ppm and 10 g/l, respectively. The highest L-serine, 24.9 g/l, was obtained at 24 h from 30.94 g/l(as dry weight) resting cells using 100 g/l initial glycine with controlled pH. The relationship between the initial rate of L-serine formation and cell concentration indicated an unusual curve due to the effects of the added NaOH which was used for controlling the pH. In similar experiments without control of pH, a normal profile was observed with respect to the relationship between the initial rate of L-serine formation and cell concentration. The highest L-serine, 54.5 g/l, was obtained at 48 h by 36.4 g/l (as dry weight) resting cells. The yield (mol of L-serine/mol of added substrate) of L-serine from methanol and glycine were 8.3% and 39.3%, respectively. The selectivity of L-serine (mol of L-serine/mol of glycine consumed) was 67.9%. The stoichiometry of the maximum L-serine formation showed that the resting cells carried the highly active methanol dehydrogenase while serine transhydroxymethylase was rather low. Serine glyoxalate aminotransferase was not completely inhibited by the high concentration of glycine (about 68% of synthesized L-serine was detected in the supernatant).
- 社団法人日本生物工学会の論文
- 1986-10-25
著者
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YAMANE TSUNEO
Laboratory of Molecular Biotechnology, Division of Molecular and Cellular Mechanisms, graduate Schoo
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Yamane Tsuneo
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Shimizu S
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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Shimizu Shoichi
Laboraotory Of Bioreaction Engineering Department Of Food Science And Technology Faculty Of Agricult
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Shiimizu Susumu
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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SIRIROTE PRAMOTE
Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agricul
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Shimizu Shoichi
Daiichi Pharmaceutical Co. Ltd.
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Yamane Tsuneo
Laboratory Of Molecular Biotechnology Graduate School Of Bio- And Agro-sciences Nagoya University
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Sirirote Pramote
Laboratory Of Bioreaction Engineering Department Of Food Science And Technology School Of Agricultur
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