Continuous Culture of CHO-K1 Cells Producing Thrombomodulin and Estimation of Culture Conditions

概要

論文の詳細を見る
Recombinant Chinese hamster ovary (CHO-K1) cells expressing human soluble thrombomodulin (rsTM) were cultured in a continuous culture system with a fluidized-bed reactor. Cells were grown in a medium containing 1% serum for 10d, and then cultured in a serum-free medium. The protein production rate increased remarkably in the serum-free culture, with a decrease in the lactate production rate. This suggests that CHO-K1 cells exhibit different physiological characteristics in response to serum remove from the medium, which resulted in a higher rsTM concentration (about 60 mg/l). A procedure for estimating protein productivity was developed using experimetal glucose and lactate measurements. In this procedure, cell density was estimated from the glucose consumption rate, and the speclfic protein (rsTM) production rate was obtained from the ratio of lactate production/glucose consumption (ΔL/ΔG). Since the cell density and protein productivity in repeated batch culture were well estimated, the procedure was applied to continuous culture in a fluidizedbed bioreactor culture. The estimation procedure was also found to be effective in this continuous culture using the models derivied from the repeated batch culture.
社団法人日本生物工学会の論文
1994-01-25