Effects of coupling method and ligand concentration on adsorption equilibrium in immuno-affinity chromatography.

スポンサーリンク

概要

• 論文の詳細を見る

• The effects of ligand concentration and coupling method on the adsorption equilibrium in immuno-affinity chromatography were studied by use of polyclonal anti-BSA antibody and Fab fragments. Immobilized IgG and Fab fragments showed similar adsorption behavior, and the adsorption equilibrium was not affected by the coupling method or the concentration of immobilized ligands. However, the binding ratio, defined as the ratio of mole antigen adsorbed to mole binding site, depended on the coupling method and decreased with increasing ligand concentration.

• 公益社団法人 化学工学会の論文

著者

• Sada Eizo

  Chemical Engineering Department Kyoto University

• KONDO AKIHIKO

  Chemical Engineering Department, Kyoto University

• Kondo Akihiko

  Chemical Engineering Department Kyoto University

• Katoh Shigeo

  Chemical Engineering Department Kyoto University

• KIYOKAWA ATSUO

  Chemical Engineering Department, Kyoto University

関連論文

• Continuous Culture of CHO-K1 Cells Producing Thrombomodulin and Estimation of Culture Conditions
• Substrate Specificity and Positional Preference of a Lipoprotein Lipase
• Effects of Light Intensity on the Growth Rate of the Red Alga Porphyridium cruentum
• HETP Values of Large-Scale Columns for Liquid Chromatography
• Lysis of Rabbit γ-Globulin-Coupled Liposomes by the Complement System
• Resistance to Proteolysis of Antibody Ligands Modified with Polyethylene Glycol
• Effective Purification of Bioproducts by Fast Flow Affinity Chromatography
• Effects of Antigen Molecular Weight on Immunoadsorption Equilibrium in Immunoaffinity Chromatography
• Effects of coupling method and ligand concentration on adsorption equilibrium in immuno-affinity chromatography.

スポンサーリンク