Purification and Refolding of Recombinant Human IGF II from Silkworms Infected with Recombinant Bombyx mori Nuclear Polyhedrosis Virus
スポンサーリンク
概要
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We have reported that insulin-like growth factor II (IGF II) was produced as a fusion protein in Bombyx mori (silkworm) larval bodies infected with recombinant B.mori nuclear polyhedrosis virus [J.Gen.Virol., 68,2599-2606 (1987)]. In this study, the purification of IGF II from the infected silkworms is reported. The fusion protein was extracted with 6.0M guanidine-HCl from the infected larval bodies homogenized in water. The use of organic solvents to remove the impurities, such as lipid derived from the larval bodies, was a very effective method of purification. IGF II was released from the partially purified fusion protein by treatment with CNBr, purified by HPLC, and refolded by air-oxidization. Refolded IGF II had an identical primary structure including disulfide bonds and showed identical thymidine uptake stimulation activity with human IGF II. Furthermore, protein disulfide-isomerase was shown to be able to refold scrambled IGF II rapidly.
- 社団法人日本農芸化学会の論文
- 1992-01-23
著者
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Numata F
Daiichi Pharmaceutical Co. Ltd. Tokyo Jpn
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MARUMOTO YASUMASA
Molecular Biology Research Laboratory
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TERUUCHI Toshiyuki
Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd.
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ENJOH Tomoko
Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd.
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NUMATA Fumio
Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd.
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SAKANO Katsu-ichi
Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Ltd.
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Enjoh Tomoko
Molecular Biology Research Laboratory Daiichi Pharmaceutical Co. Ltd.
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Sakano Katsu-ichi
Molecular Biology Research Laboratory Daiichi Pharmaceutical Co. Ltd.
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Teruuchi Toshiyuki
Molecular Biology Research Laboratory Daiichi Pharmaceutical Co. Ltd.
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Marumoto Yasumasa
Molecular Biology Research Laboratory Daiichi Pharmaceutical Co. Ltd.
関連論文
- Continuous Culture of CHO-K1 Cells Producing Thrombomodulin and Estimation of Culture Conditions
- Characteristics of CHO-K1 Cell CUlture Producing Two Types of Recombinant Soluble Thrombomodulin
- Purification and Refolding of Recombinant Human IGF II from Silkworms Infected with Recombinant Bombyx mori Nuclear Polyhedrosis Virus
- Characterization of Glycosylation on Recombinant Soluble Human Thrombomodulin Produced by Silkworms
- High-level Expression of the Human-α-interferon Gene through the Use of an Improved Baculovirus Vector in the Silkworm, Bombyx mori