Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616, a Shoyu Koji Mold, and Analysis of Its Expression

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概要

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• A xylanase gene xynGl was isolated and sequenced from Aspergillus oryzae KBN616 used for making shoyu koji. The structural part of the xynG1 gene was found to be 725 bp and was predicted to be interrupted by a single intron which was 62 bp in size. The nature XynG1 was predicted to be 189 amino acids in size and had high amino acid homology to other fungal xylanases classified in family ll glycosidase. The XynG1 was detected ill the culture supernatant of A. oryzae KBN616 grown in xylan medium but was not detected when A. oryzae KBN616 was grown in glucose medium. The xynG1 gene was introduced into A. nidulans and was found to be expressed even ill the glucose media. This expression pattern was confirmed using a luciferase gene as a reporter in A. nidulans.

• 社団法人日本生物工学会の論文
• 1998-01-25

著者

• Kojima Yuzo

  Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive

• Kajino T

  Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.

• Kimura Tetsuya

  National Center For Seeds And Seedlings

• KARITA Shuichi

  Center for Molecular Biology and Genetics Mie University

• Kitamoto Noriyuki

  Food Research Center, Aichi Industrial Technology Institute

• Kimura Tetsuya

  Laboratory of Applied Microbiology, Faculty of Bioresources, Mie University

• Sakka Kazuo

  Laboratory of Applied Microbiology, Faculty of Bioresources, Mie University

• Ohmiya Kunio

  Laboratory of Applied Microbiology, Faculty of Bioresources, Mie University

• Kojima Y

  Medical Enzyme Division Amano Enzyme Co. Ltd.

• KITO YUKIO

  Food Research Institute, Aichi Prefectural Government

• Kitamoto N

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Kito Yukio

  Food Research Institute Aichi Prefectural Government

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

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