Nucleotide Sequence of the Clostridium stercorarium xylA Gene Encoding a Bifunctional Protein with β-D-Xylosidase and α-L-Arabinofuranosidase Activities, and Properties of the Translated Product
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概要
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The nucleotides of the β-xylosidase (xylA) gene from Clostridium stercorarium were sequenced. A single open reading frame of 473 codons specifying the subunit (MW 53,340) of xylosidase was identified. The N-terminal amino acid sequence and molecular weight estimated by SDS-polyacrylamide gel electrophoresis of the purified enzyme were quite in agreement with those deduced from the nucleotide sequence. Analysis of the enzyme by gel filtration on an HPLC column gave a molecular weight of 220,000, suggesting that the native enzyme is a tetramer composed of 4 identical subunits. The pH optimum was 7.0 and quite stable over the pH range of 5 to 10 at 4℃. The optimum temperature was 65℃. V_m was estimated to be 5.9 nmol/min/μg for p-nitrophenyl-β-D-xylopyranoside and 16.7 nmol/min/μg for p-nitrophenyl-α-L-arabinofuranoside, while K_m was estimated to be 2.5 mM for p-nitrophenyl-β-D-xylopyranoside and 17.6 mM for p-nitrophenyl-α-L-arabinofuranoside.
- 社団法人日本農芸化学会の論文
- 1993-02-23
著者
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Kojima Yuzo
Medical Enzyme Division, Amano Enzyme Co., Ltd.
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Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
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YOSHIKAWA Kazuhiro
Department of Pathology, Aichi Medical University School of Medicine
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Ohmiya Kunio
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Yoshikawa K
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa Kentaro
Laboratory Of Food Microbiological Engineering Faculty Of Agriculture Kinki University
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Yoshikawa K
Department Of Bioscience Faculty Of Bioresources Mie University
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Yoshikawa Kazuhiro
Department Of Bioscience Faculty Of Bioresources Mie University
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Karita Shu-ichi
Center for Molecular Biology and Genetics, Mie University
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Shimada Kyo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department Of Bioscience Faculty Of Bioresources Mie University
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Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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Yoshida Kouji
Department Of Apple Research National Institute Of Fruit Tree Science
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YOSHIKAWA Kazuhiro
Department of Applied Chemistry, School of Engineering, Nagoya University
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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