Rice Embryo Globulins: Amino-Terminal Amino Acid Sequences, cDNA Cloning and Expression
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概要
- 論文の詳細を見る
A globulin fraction prepared from rice embryos con-tained polypeptides or polypeptide groups of 49 kDa (designated REG1), 46 kDa (designated REG2), about 35 kDa, 32 kDa and 25 kDa. The amino-terminal sequences of REG1 and the major polypeptide in the 35-kDa group were identical, suggesting that the REG1 polypeptide undergoes partial proteolytic processing that removes a carboxy-terminal region. A cDNA clone, designated pcREG2, encoding REG2 was isolated, and its nucleotide sequence was determined. The deduced amino acid sequence of REG2 was found to be 68% identical to that of the maize GLB2 globulin. Reg2 mRNA was present at high levels during embryo development for up to 14 days after flowering (DAF). Lower levels were found 20 DAF when the maturation of embryos was almost completed, and at the dry mature stage. Reg2 mRNA almost disappeared upon imbibition of isolated dry mature embryos but it was re-induced at a low level by further treatment with ABA. The expression of Reg2 was not induced by ABA in suspension-cultured cells, unlike that of Osem, one of the late embryogenesis abundant protein (LEA) genes.
- 日本植物生理学会の論文
著者
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Ohmiya Kunio
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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KARITA Shuichi
Center for Molecular Biology and Genetics Mie University
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Sun Jia-lin
Center For Molecular Biology And Genetics Mie University:department Of Bioresources Graduate School
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Nakagawa Hitoshi
Center for Molecular Biology and Genetics, Mie University
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Hattori Tsukaho
Center for Molecular Biology and Genetics, Mie University
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Sun Jia-lin
Graduate School Of Bioresources Mie Univ.
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Hattori Tsukaho
Center For Molecular Biology And Genetics Mie University:department Of Bioresources School Of Biores
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Hattori Tsukaho
Center For Molecuiar Bioiogy And Genetics Mie University
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Hattori Tsukaho
Suntory Institute For Bioorganic Research
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Nakagawa H
Fac. Horticulture. Chiba Univ.
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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Karita S
Graduate School Of Regional Innovation Studies Mie Univ.
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Karita Shuichi
Graduate School Of Bioresources Mie Univ
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Nakagawa H
National Inst. Livestock And Grassland Sci. Nishinasuno Jpn
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Sun Jialin
Graduate School Of Bioresources Mie University
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