A Cryptic Plasmid, pAO1,from a Compost Bacterium, Bacillus sp.
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概要
- 論文の詳細を見る
The complete nucleotide sequence of a new cryptic plasmid, pAO1 isolated from a compost bacterium Bacillus sp., has been analyzed. Analysis of the PCR-based 16S rRNA sequence showed the bacterium harboring pAO1 was closely related to Bacillus pallidus. The plasmid pAO1 was 3,325 bp in size. Two open reading frames, ORF1 and ORF2,encoding putative polypeptides of 248 and 290 amino acids, respectively, were identified within the sequence. The ORF1 has a limited sequence similarity to an integrase/recombinase, while the ORF2 has high similarity with the replication protein of pBC1 from Bacillus coagulans. A putative origin sequence for a plus-strand was located between ORFs. Southern blot analysis indicates this plasmid replicates via a rolling circle-type mechanism.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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KARITA Shuichi
Center for Molecular Biology and Genetics Mie University
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Karita Shuichi
Center For Molecular Biology And Genetics Mie University:(present Address)department Of Sustainable
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NAKASAKI KIYOHIKO
Department of Chemical Engineering, Faculty of Engineering, Shizuoka University.
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OHTAKI Akihito
Department of Materials Science and Chemical Engineering, Faculty of Engineering, Shizuoka Universit
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NOBORIKAWA Mina
Center for Molecular Biology and Genetics, Mie University
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Ohtaki A
Department Of Materials Science And Chemical Engineering Faculty Of Engineering Shizuoka University
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Ohtaki Akihito
Department Of Materials Science And Chemical Engineering Shizuoka University
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Noborikawa Mina
Center For Molecular Biology And Genetics Mie University
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Nakasaki Kiyohiko
Department Of Materials Science And Chemical Engineering Faculty Of Engineering Shizuoka University
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Nakasaki Kiyohiko
Department Of Chemical Engineering Faculty Of Engineering Shizuoka University
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