Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
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概要
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Chitinase B (ChiB) was purified from the culture supernatant of Xanthomonas sp. strain AK by Phenyl-Toyopearl 650M and DEAE-Toyopearl 650M column chromatographies. The purified enzyme preparation gave a single band on SDS-polyacrylamide gel electrophoresis and the molecular weight of ChiB was estimated to be 48, 000. The enzyme was optimally active at pH 6.0 and 60℃. N-Terminal amino acid sequence analysis suggested that ChiB is a member of glycosyl hydrolase family 18 and that it is genetically different from ChiA recently reported (Sakka et al., J.Ferment. Bioeng., 86, 527-533, 1998). Immunological analysis suggested that ChiB was the major chitinase species in the culture supernatant of Xanthomonas sp.strain AK and that production of the enzyme was induced by the presence of chitin.
- 公益社団法人日本生物工学会の論文
- 1999-09-25
著者
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Ohmiya Kunio
Faculty Of Bioresources Mie University
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KIMURA Tetsuya
Faculty of Bioresources, Mie University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Kimura Tetsuya
National Center For Seeds And Seedlings
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KARITA Shuichi
Center for Molecular Biology and Genetics Mie University
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Sakka Kazuo
Faculty Of Bioresources Mie University
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Hayashi Hidenori
Faculty Of Bioresources:mie University
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YAMAOKA HIDEAKI
Faculty of Bioresources, Mie University
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Yamaoka Hideaki
Faculty Of Bioresources Mie University
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Kimura Tetsuya
Faculty Of Bioresources Mie University
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