Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
スポンサーリンク
概要
- 論文の詳細を見る
The gene (egl) encoding a cellulase (EgI) from Ruminococcus albus was modified for production of the three forms of a cellulase : the pre-form having a signal sequence region, the mature form lacking the signal sequence region and a truncated form, which contained neither the signal sequence nor the N-terminal 15 amino acid residues. These modified genes were expressed in tobacco suspension cells (BY-2) under the control of cauliflower mosaic virus 35S promoter. Among the transformants harboring each type of the gene, cells having the gene encoding the truncated egI showed the strongest cellulase activity. In this transformant, 95% of the cellulase activity was located in cytoplasm and the total cellulase activity was approximately 30 times stronger than the endogenous cellulase activity in the non-transformed tobacco cells. The growth of the transformants harboring the gene encoding the trucated EgI was negligibly affected by the high level of expression of the truncated EgI.
- 公益社団法人日本生物工学会の論文
- 1996-09-25
著者
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Ohmiya Kunio
Faculty Of Bioresources Mie University
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KIMURA Tetsuya
Faculty of Bioresources, Mie University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Ohta Tsutomu
New Oji Paper Co. Ltd. Forestry Research Institute Kameyama Research Center
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Kimura Tetsuya
National Center For Seeds And Seedlings
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Sakka Kazuo
Faculty Of Bioresources Mie University
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Sakka Makiko
Graduate School Of Bioresources Mie Univ.
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Shibata M
Univ. Tokyo Tokyo Jpn
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Ito Kazuya
Kameyama Research Center Forestry Research Institute
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KAWAZU TETSU
New Oji Institute for Forest Tree Improvement
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ITO KAZUYA
New Oji Paper Co. Ltd., Forestry Research Institute, Kameyama Research Center
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SHIBATA MASARU
New Oji Paper Co. Ltd., Forestry Research Institute, Kameyama Research Center
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Kimura Tetsuya
Faculty Of Bioresources Mie University
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SHIBATA Masaru
Department of Earth and Space Science Faculty of Science, Osaka University
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SHIBATA Masaru
Graduate School of Arts and Sciences, University of Tokyo
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