Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
The Clostridium stercorarium F-9 xyl43B gene encoding the β-xylosidase Xyl43B consists of an open reading frame of 1,491 nucleotides that encodes a putative protein, classified in family 43, of 497 amino acids with a predicted molecular weight of 56,355. The deduced amino acid sequence of Xyl43B has sequence similarity with β-xylosidases from Bacteriodes thetaiotaomicron (57% sequence identity), Prevotella ruminicola (45%), Streptomyces coelicolor (40%), and Clostridium acetobutylicum (36%), all of which have been classified in family 43 of the glycoside hydrolases. Xyl43B was purified from a recombinant Escherichia coli and characterized. The optimum pH of the purified enzyme was 3.5 and it was stable over pH from 3.0 to 8.0. Its optimum temperature was 80°C and it showed thermostability in the temperature range from 50 to 70°C. Xyl43B had a Km of 6.2 mM and a Vmax of 15 μmol min−1 mg−1 for p-nitrophenyl-β-D-xylopyranoside.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
-
Ohmiya K
Graduate School Of Bioresources Mie Univ.
-
Ohmiya Kunio
Faculty Of Bioresources Mie University
-
KIMURA Tetsuya
Faculty of Bioresources, Mie University
-
Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
-
Kimura Tetsuya
National Center For Seeds And Seedlings
-
SAKKA Kazuo
Faculty of Bioresources, Mie University
-
Sun Jia-lin
Center For Molecular Biology And Genetics Mie University:department Of Bioresources Graduate School
-
Sun Jia-lin
Graduate School Of Bioresources Mie Univ.
-
Sakka Kazuo
Faculty Of Bioresources Mie University
-
Kimura Tetsuya
Graduate School Of Bioresources Mie University
-
Sun Jialin
Graduate School Of Bioresources Mie University
-
Kimura Tetsuya
Faculty Of Bioresources Mie University
関連論文
- Development of SSR Markers in Carnation (Dianthus caryophyllus)
- Round Shape Enlargement of the Yeast Spheroplast of Saccharomyces cerevisiae by HM-1 Toxin
- The Involvement of Two Specific Arginine Residues in the Action of HM-1 Killer Toxin was Deduced from Site-Directed Mutagenesis
- Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene
- Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
- Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
- Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
- Alanine-Scanning Mutagenesis of HM-1 Killer Toxin and the Essential Residues for Killing Activity
- Breeding of Yukirasha : Common Scab-Resistant Potato Variety for Table Stock
- Identification of a Catalytic Residue of Clostridium paraputrificum N-Acetyl-β-D-glucosaminidase Nag3A by Site-Directed Mutagenesis
- A Novel Thermophilic Pectate Lyase Containing Two Catalytic Modules of Clostridium stercorarium
- Detection of Hydrogen Gas-Producing Anaerobes in Refuse-Derived Fuel (RDF) Pellets
- Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)
- Functions of Family-22 Carbohydrate-Binding Module in Clostridium thermocellum Xyn10C
- Cloning of the Ruminococcus albus cel5D and cel9A Genes Encoding Dockerin Module-containing Endoglucanases and Expression of cel5D in Escherichia coli
- Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
- Analysis of Estrogen-Like Compounds in the Environment by High Performance Liquid Choromatography Bioassay (ENVIRONMENTAL BIOTECHNOLOGY)
- Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
- Hydrogen Gas Generation from Refuse-derived Fuel (RDF) under Wet Conditions
- Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)
- Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- A New Type of β-N-Acetylglucosaminidase from Hydrogen-Producing Clostridium paraputrificum M-21(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- 3D11-3 In vitro growth and hydrolysis of cellulose and xylan by Emericella nidulans Rs1 in the presence of fermentation inhibitors
- Application of Microbial Genes to Recalcitrant Biomass Utilization and Environmental Conservation
- Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells
- Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
- 613 Cloning, Sequencing and Expression of The Gene Encoding the Clostridium stercorarium Pectate Lyase Pel9A in Escherichia coli
- Characterization of Clostridium paraputrificum Chitinase A from a Recombinant Escherichia coli
- Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
- Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
- Cloning and Sequencing of a Novel Endo-1,4-β-Glucanase Gene from Ruminococcus albus
- Effect of Pressure on the Association States of Enzyme-treated Caseins
- Purification and Properties of the Endo-1,4-β-Glucanase III from Ruminococcus albus
- Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli
- Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)
- Isolation and expression analysis of phosphate transporter genes from Eucalyptus camaldulensis
- DNA Profiling of Fresh and Processed Fruits in Pear
- Identification of Quince Varieties Using SSR Markers Developed from Pear and Apple
- Identification of Asian Pear Varieties by SSR Analysis
- Thermostable β-Galactosidase from Bacillus acidocaldarius and Its Immobilization
- Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616,a Shoyu Koji Mold,and Analysis of Its Expression
- Estimation of Ploidy Levels and Breeding Backgrounds in Pot Carnation Cultivars Using Flow Cytometry and SSR Markers
- Genetic linkage map of the Japanese pear 'Housui' identifying three homozygous genomic regions
- Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers
- Integrated Reference Genetic Linkage Maps of Pear Based on SSR and AFLP Markers
- Physiological Responses to Maximal Treadmill and Deep Water Running in the Young and the Middle Aged Males
- Maximal Physiological Responses to Deep Water Running at Thermoneutral Temperature
- Purification and Characterization of the Family J Catalytic Domain Derived from the Clostridium thermocellum Endoglucanase CelJ
- Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose
- 123 Characterization of An Endoglucanase Gene from Clostridium thermocellum and Its Translated Product
- Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Product
- A Second Pectin Lyase Gene (pel2)from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Products
- Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616
- Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1
- Robust Speed Control of Ultrasonic Motor Based on H_∞ Control with Repetitive Compensator
- Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA
- Metalloregulatory Properties of the ArsR and ArsD Repressors of Acidiphilium multivorum AIU 301
- Expression of a celE Gene from Clostridium thermocellum in Bacillus
- Construction of Genetically Marked Ruminococcus albus Strains and Conjugal Transfer of Plasmid pAMβ1 into Them
- Structure of a β-Glucosidase Gene from Ruminococcus albus and Properties of the Translated Product
- Substrate Specificity of the N, 6-ο-Diacetylmuramidase from Streptomyces globisporus
- An Investigation of the pH-Activity Relationships of Cex, a Family 10 Xylanase from Cellulomonas fimi : Xylan Inhibition and the Influence of Nitro-Substituted Aryl-β-D-Xylobiosides on Xylanase Activity
- Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
- Two Proteins with Diaphorase Activity from Clostridium thermocellum and Moorella thermoacetica
- Cloning and Expression of a Clostridium kluyveri Gene Responsible for Diaphorase Activity
- Characterization of a Dihydrolipoyl Dehydrogenase Having Diaphorase Activity of Clostridium kluyveri
- Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments
- Tri-/Hexanucleotide Microsatellite Markers in Peach Derived from Enriched Genomic Libraries and Their Application in Rosaceae
- Effect of Family 22 Carbohydrate-Binding Module on the Thermostability of Xyn10B Catalytic Module from Clostridium stercorarium
- Comparison of Microbial Consortia in Refuse-Derived Fuel (RDF) Preparations between Japan and Germany
- 1G14-5 Properties of Bacillus haloduarans Xyn11A Expressed in Kluyveromyces lactis
- Different Binding Specificities of S-Layer Homology Modules from Clostridium thermocellum AncA, Slp1, and Slp2
- Enzymatic Properties of Two Catalytic Modules of Clostridium stercorarium Pectate Lyase Pel9A
- Molecular Cloning of Xylanase Gene xynG1 from Aspergillus oryzae KBN 616, a Shoyu Koji Mold, and Analysis of Its Expression
- Productino of HM-1 Killer Toxin in Saccharomyces cerevisiae Transformed with the PDR4 Gene and δ-Sequence-Mediated Multi-Integration System
- The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
- Structural Relationships among Killer Toxins Secreted from the Killer Strains of the Genus Williopsis
- Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
- Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
- Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
- Sequence of egV and Properties of EgV, a Ruminococcus albus Endoglucanase Containing a Dockerin Domain
- Process of Thermal Denaturation of Xylanase (XynB) from Clostridium stercorarium F-9
- Expression of a Bacterial Endoglucanase Gene in Tobacco Increases Digestibility of Its Cell Wall Fibers
- Adsorption of Clostridium stercorarium Xylanase A to Insoluble Xylan and the Importance of the CBDs to Xylan Hydrolysis
- Functions of Family-22 Carbohydrate-Binding Modules in Clostridium josui Xyn10A
- Function of the Family-9 and Family-22 Carbohydrate-Binding Modules in a Modular β-1,3-1,4-Glucanase/Xylanase Derived from Clostridium stercorarium Xyn10B
- Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
- Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
- Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells
- Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21
- Phylogeography of the endangered tideland snail Batillaria zonalis in the Japanese and Ryukyu Islands
- Phylogenetic Relationships between the Tideland Snails Batillaria flectosiphonata in the Ryukyu Islands and B. multiformis in the Japanese Islands(Animal Diversity and Evolution)
- Identification and Characterization of Clostridium paraputrificum M-21, a Chitinolytic, Mesophilic and Hydrogen-Producing Bacterium
- Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
- Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
- Cloning, Sequencing, and Expression of the Gene Encoding a Cell-bound Multi-domain Xylanase from Clostridium josui, and Characterization of the Translated Product
- Molecular Breeding of Cellulolytic Microbes, Plants, and Animals for Biomass Utilization
- Cloning of the celB Gene Encoding Endo-1,4-β-Glucanase-2 from Clostridium josui in Escherichia coli and the Properties of the Translated Product
- Purification and Characterization of the Clostridium josui Porphobilinogen Deaminase Encoded by the hemC Gene from a Recombinant Escherichia coil
- Nucleotide Sequence of the Clostridium stercorarium xynB Gene Encoding an Extremely Thermostable Xylanase, and Characterization of the Translated Product