Cloning of the celB Gene Encoding Endo-1,4-β-Glucanase-2 from Clostridium josui in Escherichia coli and the Properties of the Translated Product
スポンサーリンク
概要
- 論文の詳細を見る
The gene celB on a 3.9-kilobase-pair (kbp) EcoRI fragment encoding endo-1,4-β-glucanase of Clostridium josui was cloned into Escherichia coli. The structural gene located on the 1.6 kbp Sau3AI fragment excised from the EcoRI fragment was expressed by the lac promoter in the transformant E. coli JM103 (pUCJ2) in modified Luria-Bertani broth with an activity 1,000 times (1120 U/l) higher than that on the EcoRI fragment. The translation product of celB in pUCJ2 was purified by CM Bio-Gel A column chromatography. The homogeneous enzyme was 42 kD of the molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optima for the temperature and pH of the enzyme were 60℃ and 5.5,respectively. The enzyme hydrolyzed cellotetraose, cellopentaose and cellohexaose but not cellobiose and cellotriose. The mode of degradation of cellooligomers (G4→2G2,G5→G2+G3,G6→2G3) of the enzyme suggested that it acts as an endo-1,4-β-glucanase. This endoglucanase is distinguishable from those characterized by us previously with respect to its pH optimum and cellobiose-transferring activity.
- 公益社団法人日本生物工学会の論文
- 1991-12-25
著者
-
Ohmiya Kunio
Faculty Of Bioresources Mie University
-
Fujino Tsuchiyoshi
Bio Development Division Nagoya Seiraku Co. Ltd.
関連論文
- Molecular Cloning, Characterization, and Expression Analysis of the xynF3 Gene from Aspergillus oryzae(Microbiology & Fermentation Technology)
- Molecular Cloning, Overexpression, and Purification of a Major Xylanase from Aspergillus oryzae
- Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
- Identification of a Catalytic Residue of Clostridium paraputrificum N-Acetyl-β-D-glucosaminidase Nag3A by Site-Directed Mutagenesis
- A Novel Thermophilic Pectate Lyase Containing Two Catalytic Modules of Clostridium stercorarium
- Detection of Hydrogen Gas-Producing Anaerobes in Refuse-Derived Fuel (RDF) Pellets
- Promoter of Arabidopsis thaliana Phosphate Transporter Gene Drives Root-Specific Expression of Transgene in Rice(Plant Biotechnology)
- Functions of Family-22 Carbohydrate-Binding Module in Clostridium thermocellum Xyn10C
- Cloning of the Ruminococcus albus cel5D and cel9A Genes Encoding Dockerin Module-containing Endoglucanases and Expression of cel5D in Escherichia coli
- Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
- Analysis of Estrogen-Like Compounds in the Environment by High Performance Liquid Choromatography Bioassay (ENVIRONMENTAL BIOTECHNOLOGY)
- Sequencing and Expression of the Gene Encoding the Clostridium stercorarium β-Xylosidase Xyl43B in Escherichia coli
- Hydrogen Gas Generation from Refuse-derived Fuel (RDF) under Wet Conditions
- Cloning, Sequencing, and Expression of the Gene Encoding the Clostridium stercorarium α-Galactosidase Aga36A in Escherichia coli(Microbiology & Fermentation Technology)
- Electrotransformation of Clostridium paraputrificum M-21 with Some Plasmids(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- A New Type of β-N-Acetylglucosaminidase from Hydrogen-Producing Clostridium paraputrificum M-21(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Application of Microbial Genes to Recalcitrant Biomass Utilization and Environmental Conservation
- Stable Expression of a Thermostable Xylanase of Clostridium thermocellum in Cultured Tobacco Cells
- Isothermal Titration Calorimetric Studies on the Binding of a Family 6 Carbohydrate-binding Module of Clostridium thermocellum XynA with Xlylooligosaccharides(Biochemistry & Molecular Biology)
- Characterization of Clostridium paraputrificum Chitinase A from a Recombinant Escherichia coli
- Cloning, DNA Sequencing, and Expression of the β1,4-Mannanase Gene from a Marine Bacterium, Vibrio sp. Strain MA-138
- Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product
- Purification and Properties of the Endo-1,4-β-Glucanase III from Ruminococcus albus
- Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli
- Antimutagenic Effects of Ajoene, an Organosulfur Compound Derived from Garlic
- Thermostable β-Galactosidase from Bacillus acidocaldarius and Its Immobilization
- 123 Characterization of An Endoglucanase Gene from Clostridium thermocellum and Its Translated Product
- Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Product
- A Second Pectin Lyase Gene (pel2)from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Products
- Sequence Analysis and Overexpression of a Pectin Lyase Gene (pel1) from Aspergillus oryzae KBN616
- Purification and Characterization of the Overexpressed Aspergillus oryzae Xylanase, XynF1
- Cloning and Sequencing of the Gene Encoding Chitinase ChiA from Xanthomonas sp.Strain AK and Some Properties of ChiA
- Expression of a celE Gene from Clostridium thermocellum in Bacillus
- Construction of Genetically Marked Ruminococcus albus Strains and Conjugal Transfer of Plasmid pAMβ1 into Them
- Structure of a β-Glucosidase Gene from Ruminococcus albus and Properties of the Translated Product
- An Investigation of the pH-Activity Relationships of Cex, a Family 10 Xylanase from Cellulomonas fimi : Xylan Inhibition and the Influence of Nitro-Substituted Aryl-β-D-Xylobiosides on Xylanase Activity
- The Nitrate Reductase Gene from a Shoyu Koji Mold, Aspergillus oryzae KBN616
- Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201
- Purification, Characterization, and Molecular Cloning of Acidophilic Xylanase from Penicillium sp.40
- Characterization of the Cellulolytic Complex (Cellulosome) from Ruminococcus albus
- Sequence of egV and Properties of EgV, a Ruminococcus albus Endoglucanase Containing a Dockerin Domain
- Process of Thermal Denaturation of Xylanase (XynB) from Clostridium stercorarium F-9
- Expression of a Bacterial Endoglucanase Gene in Tobacco Increases Digestibility of Its Cell Wall Fibers
- Adsorption of Clostridium stercorarium Xylanase A to Insoluble Xylan and the Importance of the CBDs to Xylan Hydrolysis
- Importance of the Carbohydrate-Binding Module of Clostridium stercorarium Xyn10B to Xylan Hydrolysis
- Conversion of Chitinous Wastes to Hydrogen Gas by Clostridium paraputrificum M-21
- Identification and Characterization of Clostridium paraputrificum M-21, a Chitinolytic, Mesophilic and Hydrogen-Producing Bacterium
- Expression of a Ruminococcus albus Cellulase Gene in Tobacco Suspension Cells
- Purification and Some Properties of a Chitinase from Xanthomonas sp. Strain AK
- Cloning, Sequencing, and Expression of the Gene Encoding a Cell-bound Multi-domain Xylanase from Clostridium josui, and Characterization of the Translated Product
- Molecular Breeding of Cellulolytic Microbes, Plants, and Animals for Biomass Utilization
- Nucleotide Sequence of an Endo-1,4-β-Glucanase Gene (celA) from Clostridium josui
- Cloning of the celB Gene Encoding Endo-1,4-β-Glucanase-2 from Clostridium josui in Escherichia coli and the Properties of the Translated Product
- Purification and Characterization of the Clostridium josui Porphobilinogen Deaminase Encoded by the hemC Gene from a Recombinant Escherichia coil
- Nucleotide Sequence of the Clostridium stercorarium xynB Gene Encoding an Extremely Thermostable Xylanase, and Characterization of the Translated Product