Pectin Methylesterase Gene (pmeA) from Aspergillus oryzae KBN616 : Its Sequence Analysis and Overexpression, and Characterization of the Gene Product

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概要

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• A gene (pmeA) encoding pectin methylesterase was isolated from a shoyu koji mold, Aspergillus oryzae KBN616,and characterized. The structural gene comprised 1,370 bp with six introns. The PMEA protein consisted of 331 amino acids with a putative signal peptide of 17 amino acids. The deduced amino acid sequence was very similar to those of Aspergillus niger PMEA and Aspergillus aculeatus PME1. The pmeA gene was efficiently expressed under control of the A. oryzae TEF1 gene promoter for purification and characterization of the ezymatic properties. PMEA had a molecular mass of 38.5 kDa, a pH optimum of 5.0,and a temperature optimum of 55℃.

• 社団法人日本農芸化学会の論文
• 1999-01-23

著者

• Ohmiya Kunio

  Faculty Of Bioresources Mie University

• Okada Hideki

  Research And Development Center Cosmo Oil Co. Ltd.

• Kitamoto Noriyuki

  Food Research Center, Aichi Industrial Technology Institute

• TSUKAGOSHI Norihiro

  Department of Food Science and Technology, Faculty of Agriculture, Nagoya University

• Okada Hideki

  Shin Nihon Chemical Co. Ltd

• YOSHINO Shoko

  Food Research Institute, Aichi Prefectural Government

• Yoshino Shoko

  Food Research Institute Aichi Prefectural Government

• Kitamoto N

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Tsukagoshi N

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tsukagoshi Norihiro

  Department Of Applied Biological Sciences Faculty Of Agriculture Nagoya University

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

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