Development of a Highly Efficient Gene Replacement System for an Industrial Strain of Aspergillus oryzae Used in the Production of Miso, a Japanese Fermented Soybean Paste

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概要

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• A homologous gene replacement system was developed for an industrial strain of Aspergillus oryzae KBN630 used in miso production. This system was based on a transformation method involving a bidirectional marker gene, pyrG. To increase the integration of exogenous DNA fragments by homologous recombination, the ku70 gene encoding the Ku70 component of the nonhomologous end-joining machinery was removed from the genome of a pyrG deletion mutant derived from A. oryzae KBN630. The efficiency of homologous integration at the amyR locus was more than 90%.

• 2011-03-01

著者

• Kato Masashi

  Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Hasegawa Osamu

  Food Research Center Aichi Industrial Technology Institute

• Mori Akihiro

  Food Research Center Aichi Industrial Technology Institute

• YOSHINO YASUDA

  Food Research Center, Aichi Industrial Technology Institute

• ISHIHARA Nami

  Food Research Center, Aichi Industrial Technology Institute

• Yoshino Yasuda

  Food Research Center Aichi Industrial Technology Institute

• Ishihara Nami

  Food Research Center Aichi Industrial Technology Institute

• Kitamoto Noriyuki

  Aichi Industrial Technol. Inst. Aichi Jpn

• Kato Masashi

  Department Of Applied Biological Chemistry Fuculty Of Agriculture Meijo University

• Kato Masashi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

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