In Vivo and in Vitro Analyses of the AmyR Binding Site of the Aspergillus nidulans agdA Promoter ; Requirement of the CGG Direct Repeat for Induction and High Affinity Binding of AmyR(Biochemistry & Molecular Biology)

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The α-glucosidase gene (agdA) of Aspergillus nidulans has a single CGGN_8CGG type AmyR binding site in its promoter region. The binding site is functional in vivo as a cis-element responsible for induction by starch, and mutational studies indicated that both the CGG triplets are required for high-level induction. A part of AmyR (residues 1-411 ; AmyR_<1-411>), which was produced as a MalE fusion protein in E. coli, bound to the CGGN_8CGG site of the agdA promoter. DNA binding profiles to the mutant binding sites that lacked both or either one of the CGG triplets suggested that AmyR_<1-411> can bind to a single CGG triplet site with low affinity and that two AmyR molecules cooperatively bind to the CGG direct repeat.
社団法人日本農芸化学会の論文
2001-07-23