Coexistence of Two Kinds of 6-Phospho-β-Galactosidase in the Cytosol of Lactobacillus gasseri JCM1031

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概要

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• Lactobacillus (L.) gasseri JCM1031 has 2 kinds of 6-phospho-β-galactosidase (P-β-gal) in the cytosol. P-β-gal I was already isolated and characterized for our previous paper [Biosci. Biotech. Biochem.,60, 139-141 (1996)]. Another, P-β-gal II, was purified to homogeneity through several liquid chromatographic steps for this paper. The molecular mass of the purified enzyme was estimated to be 58 kDa by SDS-PAGE. The K_m and V_<max> for ONPGal-6P were 0.76 mM and 86.0 μmol/min/mg, respectively. Reducing reagents and Fe^<2+> ion activated the enzyme, but PCMB, Zn^<2+>, and Hg^<2+> ions, and alkylation reagents strongly inihibited it. Twenty-five residues of the N-terminal amino acid sequence of the enzyme were identified. P-β-gal II was different from P-β-gal I in several characteristics.

• 社団法人日本農芸化学会の論文
• 1996-04-23

著者

• Suzuki Megumi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• SAITO Tadao

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• Itoh Tomoyuki

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Suzuki M

  Yamagata Univ. Yamagata Jpn

• ITOH Takatoshi

  Laboratory of Animal Food Science, College of Bioresource Science, Nihon University

• Itoh T

  Laboratory Of Animal Food Science College Of Bioresource Science Nihon University

• Suzuki Masakatsu

  Laboratory Of Animal Products Chemistry

• Itoh T

  Faculty Of Agriculture Iwate University

• Itoh T

  Hiroshima Univ. Higashi-hiroshima Jpn

• Saito Tadao

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

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