Chitinases A, B, and C1 of Serratia marcescens 2170 Produced by Recombinant Escherichia coli : Enzymatic Properties and Synergism on Chitin Degradation(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
To discover the individual roles of the chitinases from Serratia marcescens 2170, chitinases A, B, and C1 (ChiA, ChiB, and ChiC1) were produced by Escherichia coli and their enzymatic properties as well as synergistic effect on chitin degradation were studied. All three chitinases showed a broad pH optimum and maintained significant chitinolytic activity between pH 4 and 10. ChiA was the most active enzyme toward insoluble chitins, but ChiC1 was the most active toward soluble chitin derivatives among the three chitinases. Although all three chitinases released (GlcNAc)_2 almost exclusively from colloidal chitin, ChiB and ChiC1 split (GlcNAc)_6 to (GlcNAc)_3, while ChiA exclusively generated (GlcNAc)_2 and (GlcNAc)_4. Clear synergism on the hydrolysis of powdered chitin was observed in the combination between ChiA and either ChiB or ChiC, and the sites attacked by ChiA on the substrate are suggested to be different from those by either ChiB or ChiC1.
- 社団法人日本農芸化学会の論文
- 2002-05-23
著者
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Watanabe Takashi
Department of Chemistry, Graduate School of Science, Kyoto University
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SUGAWARA NORIKO
Department of Pediatrics, Tohoku University Graduate School of Medicine
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Suzuki Kazuharu
Department Of Nutritional Science Tokyo University Of Agriculture
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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Suzuki Megumi
Department Of Cardiology Fujita Health University
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Suzuki Megumi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki K
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki M
Yamagata Univ. Yamagata Jpn
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Suzuki K
Univ. Tokyo Tokyo
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Suzuki K
Taisho Pharmaceutical Co. Ltd. Saitama Jpn
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Suzuki Kazushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University:(present Addres
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Suzuki Kazushi
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Suzuki K
Department Of Nutritional Science Tokyo University Of Agriculture
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UCHIYAMA Taku
Department of Biosystem Science, Graduate School of Science and Technology, Niigata University
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NIKAIDOU Naoki
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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KATOUNO Fuminori
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Suzuki Masakatsu
Laboratory Of Animal Products Chemistry
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Watanabe T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki K
Laboratory Of Molecular Structure And Function Department Of Molecular Biology Institute Of Molecula
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Nikaidou N
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Nikaidou Naoki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sugawara N
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Sugawara Noriko
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sakurai K
Department Of Nutritional Science Tokyo University Of Agriculture
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Uchiyama Taku
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki Kazuharu
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Katouno Fuminori
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Watanabe Takashi
Department Of Cardiothoracic Surgery Nagoya University School Of Medicine
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Watanabe Takashi
Department Of Applied Physics Tohoku University
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