<Short Communication>Characterization of Arginine as a Receptor Component for Coliphage T2H and Regions for Production of Phage Virion
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概要
- 論文の詳細を見る
The number of phage T2H particles decreased with an increase in the amounts of authentic D-arginine and the lipoprotein preparation from Escherichia coli B. The number of phage particles, preincubated with D-arginine or lipoprotein preparation, did not decrease with the addition of indole which inhibits the adsorption of phage T2H to the host cell. These results suggest that D-arginine defines the characteristics of the adsorption of phage T2H or occupies the determinant group of the receptor site for the phage on the cell surface of E. coli B. The radioactivity of [^3H]-thymidine-labeled phage T2H DNA was coincident with the NADH oxidase activity in the inner membrane fraction from the cell envelope of E. coli B, by centrifugation in a sucrose density gradient. It is quite possible that the phage DNA which binds to the receptor site on lipoprotein moiety in the outer membrane may reach specific loci on the inner membrane within 20 min of a latent period, after which production of the phage virion would occur.
- 島根大学の論文
著者
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Watanabe Takashi
Department of Chemistry, Graduate School of Science, Kyoto University
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SUGIYAMA Akira
Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, U
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SAITO Hajime
Department of Life Science, Himeji Institute of Technology, Harima Science Garden City
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Saito Hajime
Department Of Microbiology And Immunology Shimane Medical University
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ASANO KYOKO
Department of Microbiology and Immunology, Shimane Medical University
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TADO OSAMU
Department of Urology, Shimane Medical University
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Asano Kyoko
Department Of Microbiology And Immunology Shimane Medical University
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Tado Osamu
Department Of Urology Shimane Medical University
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Saito Hajime
Department Of Biology Faculty Of Science Okayama University
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Sugiyama Akira
Department Of Electronic Engineering Mining College Akita University
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Sugiyama Akira
Department Of Microbiology Mie University School Of Medicine
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Watanabe Takashi
Department Of Cardiothoracic Surgery Nagoya University School Of Medicine
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Watanabe Takashi
Department Of Applied Physics Tohoku University
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Sugiyama Akira
Department of Chemical System Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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WATANABE TAKASHI
Department of Microbiology and Immunology, Shimane Medical University
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Saito Hajime
Department of Applied Chemistry, Faculty of Engineering, Nagoya University
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