Isolation and Characterization of a Highly Hydrophobic New Bacteriocin (Gassericin A) from Lactobacillus gasseri LA39

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概要

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• A new bacteriocin, gassericin A, was purified from the culture fluid of Lactobacillus gasseri LA39 mainly by reverse-phase (RP) chromatography. The purification of gassericin A from a modified MRS broth, in which Tween 80 had been replaced by oleic acid, resulted in a 4500-fold increase in specific activity with a 6% recovery. Gassericin A was eluted as a single peak on the chromatogram from RP-HPLC and migrated by SDS-PAGE as a single band with a molecular weight of ca. 3. 8 kDa. Gassericin A, a highly hydrophobic bacteriocin, was slightly soluble in water, but its solubility was increased by adding alcohol and acetonitrile. An amino acid analysis revealed that it was composed of 45.7% hydrophobic amino acids in the total residues of 35 amino acids. Gassericin A produced in the MRS broth associated strongly with Tween 80, although several further trials of dissociation were unsuccessful.

• 社団法人日本農芸化学会の論文
• 1994-07-23

著者

• SAITO Tadao

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• Itoh Tomoyuki

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• KAWAI Yasushi

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• ITOH Takatoshi

  Laboratory of Animal Food Science, College of Bioresource Science, Nihon University

• Itoh T

  Laboratory Of Animal Food Science College Of Bioresource Science Nihon University

• Itoh T

  Faculty Of Agriculture Iwate University

• Itoh T

  Hiroshima Univ. Higashi-hiroshima Jpn

• TOBA Takahiro

  Laboratory of Animal Products Technology, Faculty of Agriculture, Tohoku University

• Toba T

  Laboratory Of Animal Products Chemistry College Of Agriculture

• Toba Takahiro

  Laboratory Of Animal Products Technology College Of Agriculture Tohoku University

• Samant K.

  Laboratory of Animal Products Chemistry, Faculty of Agriculture, Tohoku University

• Samant K.

  Laboratory Of Animal Products Chemistry Faculty Of Agriculture Tohoku University

• Saito Tadao

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

• Toba Takahiro

  Laboratory of Animal Products Chemistry, College of Agriculture

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