Sequence Analysis by Cloning of the Structural Gene of Gassericin A, a Hydrophobic Bacteriocin Produced by Lactobacillus gasseri LA39

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概要

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• Gassericin A, a bacteriocin from Lactobacillus gasseri LA39,was purified to homogeneity from the culturesupernatant mainly by reverse-phase chromatography. The molecular weight of gassericin A was found to be 5,652 by mass analysis, unlike the estimated 3,800 found by SDS-PAGE. However, when the purified preparation was treated with lysylendopeptidase, it migrated as a single band to 5,600 with bacteriocin activity on SDS-PAGE. N-and C-terminal amino acids could not be identified. The internal amino acid could be identified after gassericin A was hydrolyzed with lysylendopeptidase. The DNA of the structural gene of gassericin A was sequenced by cloning of the gene from chromosomal DNA with an oligonucleotide probe. The structural gene of gassericin A was found on the chromosomal DNA as an open reading frame encoding a protein composed of 91 amino acids. The amino acid sequence of mature gassericin A was predicted to be 58 residues from the DNA sequence and results of mass analysis. These results suggested that gassericin A has a closed circular structure with N-and C-terminals linked. Gassericin A is a hydrophobic class II bacteriocin ; it was 98% identical with acidocin B produced by Lactobacillus acidophillus M46.

• 社団法人日本農芸化学会の論文
• 1998-05-23

著者

• Kawai Yasushi

  東北大学 農学研究科

• Suzuki Megumi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• SAITO Tadao

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• Itoh Tomoyuki

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• KAWAI Yasushi

  Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

• Suzuki M

  Yamagata Univ. Yamagata Jpn

• ITOH Takatoshi

  Laboratory of Animal Food Science, College of Bioresource Science, Nihon University

• Itoh T

  Laboratory Of Animal Food Science College Of Bioresource Science Nihon University

• Suzuki Masakatsu

  Laboratory Of Animal Products Chemistry

• Itoh T

  Faculty Of Agriculture Iwate University

• Itoh T

  Hiroshima Univ. Higashi-hiroshima Jpn

• Suzuki Masakazu

  Laboratory Of Forest Hydrology And Erosion Control Engineering Graduate School Of Agricultural And L

• Saito Tadao

  Laboratory Of Animal Products Chemistry Graduate School Of Agricultural Science Tohoku University

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