Assembly of Staphylococcus aureus Leukocidin into a Pore-Forming Ring-Shaped Oligomer on Human Polymorphonuclear Leukocytes and Rabbit Erythrocytes

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概要

• 論文の詳細を見る

• Staphylococcal leukocidin consists of two separate proteins, LukS and LukF, which cooperatively lyse human and rabbit polymorphonuclear leukocytes and rabbit erythrocytes. Here we studied the pore-forming properties of leukocidin and the molecular architecture of the leukocidin pore. (1) Leukocidin caused an efflux of potassium ions from rabbit erythrocytes and swelling of the cells before hemolysis. However, ultimate lysis of the toxin-treated swollen erythrocytes did not occur when polyethylene glycols with hydrodynamic diameters of ≥2.1 nm were present in the extracellular space. (2) Electron microscopy showed the presence of a ring-shaped structure with outer and inner diameters of 9 and 3 nm, respectively, on leukocidin-treated human polymorphonuclear leukocytes and rabbit erythrocytes. (3) Ring-shaped structures of the same dimensions were isolated from the target cells, and they contained LukS and LukF in a molar ratio of 1 : 1. (4) A single ring shaped toxin complex had a molecular size of 205 kDa. These results indicated that LukS and LukF assemble into a ring-shaped oligomer of approximately 200 kDa on the target cells, forming a membrane pore with a functional diameter of approximately 2 nm.

• 社団法人日本農芸化学会の論文
• 1999-05-23

著者

• Kamio Yoshiyuki

  Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric

• Kawai Yasushi

  東北大学 農学研究科

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• SUGAWARA Noriko

  Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agr

• TOMITA Toshio

  Laboratory of the Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• SATO Tsuruji

  Division of Electron Microscopy, Graduate School of Agricultural Science, Tohoku University

• Tomita T

  Showa Sangyo Co. Ltd.

• Sugawara N

  Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric

• Sugawara Noriko

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Kamio Yoshiyuki

  Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

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