Prophage, φPV83-pro, Carrying Panton-Valentine Leukocidin Genes, on the Staphylococcus aureus P83 Chromosome : Comparative Analysis of the Genome Structures of φPV83-pro, φPVL, φ11,and Other Phages
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概要
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Staphylococcus aureus P83 has Panton-Valentine leukocidin (PVL)-like genes, lukM and lukF-PV. Here, lukM and lukF-PV genes were found on the genome of a prophage, which was designated as φPV83-pro. The precise genome size was 45,636 bp with att core sequences of 10 base pairs. Sixty-four ORFs were identified on the φPV83-pro genome, including two extra operons, lukM-lukF-PV and orfs63-64. The lukM-lukF-PV cluster was located 2.1 kb upstream of the attL site. The most striking feature of the φPV83-pro genome was a constituent of at least 4 regions from φ11,φPVL, and other phages, i.e., (i) att sites identical with those of φ11,(ii) a cos sequence and the genes encoding packaging and head proteins of φPVL (occupied half region of φPV83-pro), and (iii) the other two regions which showed no significant similarity with known phages (occupied about 40% of φPV83-pro). Furthermore, two insertion sequences, ISSA1 and ISSA2 were integrated into attL site and orf44,respectively. φPV83-pro was not induced as phage particles from S. aureus P83 regardless of its treatment with mitomycin C. The insertion of ISSA1 into the attL site was one of the reasons of the failure of the induction of the phage particles by mitomycin C treatment of the strain P83.
- 社団法人日本農芸化学会の論文
- 2000-12-23
著者
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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KANEKO Jun
Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Division of Bioscience an
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Kaneko Jun
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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Kamio Yoshiyuki
Laboratory Of Applied Microbiology Department Of Microbial Biotechnology Graduate School Of Agricult
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ZOU Dan
Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agr
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NARITA Sachiko
Laboratory of Applied Microbiology, Department of Molecular and Cell Biology, Graduate School of Agr
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Zou Dan
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Narita Sachiko
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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Kaneko Jun
Laboratory Of Applied Microbiology Department Of Molecular And Cell Biology Graduate School Of Agric
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