Isolation and Characterization of the Glyceraldehyde-3-phosphate Dehydrogenase Gene of Lentinus edodes

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概要

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• The glyceraldehyde-3-phosphate dehydrogenase (GPD) gene of Lentinus edodes was isolated from a genomic DNA library and cDNA corresponding to this gene was isolated from a mycelium cDNA library. The L. edodes GPD gene was found to encode a 337-aa protein. By comparison of the cDNA and genomic DNA sequences, the presence of eight introns in the GPD gene was confirmed. The putative amino acid sequence of the L. edodes GPD gene product showed high similarity to those of other basidiomycetes. The results of Southern blot analyses suggested that only one copy of the GPD gene is present in the genome of L. edodes. The promoter region was found to contain a CT-rich stretch, two CAAT boxes and a consensus TATA box. In addition, the transcript of the GPD gene was found to be expressed constitutively and strongly. These results suggest that the promoter of the L. edodes GPD gene may be very useful as a component of transformation vectors.

• 社団法人日本農芸化学会の論文
• 1999-07-23

著者

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Hirano T

  Kyoto Univ. Kyoto Jpn

• Enei H

  Iwate Biotechnol. Res. Center Iwate Jpn

• Enei Hitoshi

  Iwate Biotechnology Research Center

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Yaegashi Kaori

  Iwate Biotechnology Res. Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• Enei H

  Iwate Biotechnology Research Center

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• OKAWA Kumiko

  Iwate Biotechnology Research Center

• HIRANO Tatsuya

  Iwate Biotechnology Research Center

• KANDA Katsuhiro

  Iwate Biotechnology Research Center

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Taniyama Hiroko

  Department Of System Engineering Of Materials And Life Science Faculty Of Engineering Toyama Univers

• Horikawa Takashi

  Collaborative Research Center For Advanced Science And Technology Osaka University

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

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